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The binding of 3H-prostaglandin E2 (PGE2) to rabbit gastric mucosa was investigated. Binding depended on incubation time, temperature and pH, and was saturable and reversible. Scatchard plot analysis revealed a single class of binding sites with a dissociation constant (Kd) of 5.33 +/- 0.21 nM and a maximum number of binding sites (Bmax) of 138.1 +/- 3.4 fmol/mg protein. PGE1 and 16,16-dimethyl PGE2 potently competed with 3H-PGE2 for the binding sites of gastric mucosa, whereas PGA2, PGF2 alpha, 6-keto PGF1 alpha and thromboxane B2 were less potent. The gastric mucosa prepared from the rabbits given indomethacin (5 mg/kg s.c. three times) showed a lower Kd (2.47 +/- 0.19 nM) for 3H-PGE2 than that from untreated one. Treatment with a PGE1 analog, misoprostol (320 micrograms/kg s.c. three times) lowered the Bmax to 74.1 +/- 2.4 fmol/mg protein without any significant effect on the Kd value. It is concluded that rabbit gastric mucosa has specific binding sites for 3H-PGE2 which may be modulated by the levels of PGs in vivo.
M Tomoi, and
M Matsuo, and
T Ono, and
F Shibayama
March 1983,
Prostaglandins,
M Tomoi, and
M Matsuo, and
T Ono, and
F Shibayama
September 1984,
Arkhiv anatomii, gistologii i embriologii,
M Tomoi, and
M Matsuo, and
T Ono, and
F Shibayama
May 1986,
Canadian journal of physiology and pharmacology,
M Tomoi, and
M Matsuo, and
T Ono, and
F Shibayama
October 1979,
Gut,
M Tomoi, and
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F Shibayama
October 1981,
The American journal of physiology,
M Tomoi, and
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F Shibayama
November 1988,
The Journal of physiology,
M Tomoi, and
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T Ono, and
F Shibayama
July 1983,
Biokhimiia (Moscow, Russia),
M Tomoi, and
M Matsuo, and
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F Shibayama
January 1986,
General pharmacology,
M Tomoi, and
M Matsuo, and
T Ono, and
F Shibayama
March 1987,
Arthritis and rheumatism,
M Tomoi, and
M Matsuo, and
T Ono, and
F Shibayama
August 1998,
The Journal of pharmacology and experimental therapeutics,
