-transparent.png){kind=logo}
The DNA methylome of human peripheral blood mononuclear cells. 2010
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
BGI-Shenzhen, Shenzhen, Guangdong, China.
DNA methylation plays an important role in biological processes in human health and disease. Recent technological advances allow unbiased whole-genome DNA methylation (methylome) analysis to be carried out on human cells. Using whole-genome bisulfite sequencing at 24.7-fold coverage (12.3-fold per strand), we report a comprehensive (92.62%) methylome and analysis of the unique sequences in human peripheral blood mononuclear cells (PBMC) from the same Asian individual whose genome was deciphered in the YH project. PBMC constitute an important source for clinical blood tests world-wide. We found that 68.4% of CpG sites and <0.2% of non-CpG sites were methylated, demonstrating that non-CpG cytosine methylation is minor in human PBMC. Analysis of the PBMC methylome revealed a rich epigenomic landscape for 20 distinct genomic features, including regulatory, protein-coding, non-coding, RNA-coding, and repeat sequences. Integration of our methylome data with the YH genome sequence enabled a first comprehensive assessment of allele-specific methylation (ASM) between the two haploid methylomes of any individual and allowed the identification of 599 haploid differentially methylated regions (hDMRs) covering 287 genes. Of these, 76 genes had hDMRs within 2 kb of their transcriptional start sites of which >80% displayed allele-specific expression (ASE). These data demonstrate that ASM is a recurrent phenomenon and is highly correlated with ASE in human PBMCs. Together with recently reported similar studies, our study provides a comprehensive resource for future epigenomic research and confirms new sequencing technology as a paradigm for large-scale epigenomics studies.
Related Publications
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
July 2004,
Biochemical and biophysical research communications,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
April 1991,
American journal of clinical pathology,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
August 2007,
Current protocols in microbiology,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
January 1979,
Journal of immunological methods,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
October 2015,
Clinical and experimental immunology,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
March 2012,
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
October 1998,
Journal of autoimmunity,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
November 2019,
Journal of medical virology,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
August 2019,
Journal of clinical medicine,
Yingrui Li, and
Jingde Zhu, and
Geng Tian, and
Ning Li, and
Qibin Li, and
Mingzhi Ye, and
Hancheng Zheng, and
Jian Yu, and
Honglong Wu, and
Jihua Sun, and
Hongyu Zhang, and
Quan Chen, and
Ruibang Luo, and
Minfeng Chen, and
Yinghua He, and
Xin Jin, and
Qinghui Zhang, and
Chang Yu, and
Guangyu Zhou, and
Jinfeng Sun, and
Yebo Huang, and
Huisong Zheng, and
Hongzhi Cao, and
Xiaoyu Zhou, and
Shicheng Guo, and
Xueda Hu, and
Xin Li, and
Karsten Kristiansen, and
Lars Bolund, and
Jiujin Xu, and
Wen Wang, and
Huanming Yang, and
Jian Wang, and
Ruiqiang Li, and
Stephan Beck, and
Jun Wang, and
Xiuqing Zhang
June 2018,
Clinical laboratory,
