Biomaterial Database

A competitive ELISA for the detection of group-specific antibodies to African horse sickness virus. 1990

C Hamblin, and S D Graham, and E C Anderson, and J R Crowther

Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey.

A competition enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid identification and quantification of antibodies against African horse sickness (AHS) in sera from solipeds. The data showed the ELISA to be sensitive, specific and reliable. More than 1600 sera from 37 different countries were examined and results compared with those obtained by agar gel immuno-diffusion (AGID) tests. In no case did any of 775 sera from countries where AHS has never been reported and where AHS vaccines are not used, record an ELISA titre greater than 4. A titre equal to or greater than 8 was considered positive. Using this criterion, 96.3% of sera tested in both assays were in agreement. Doubtful results by AGID (1.7%) were clearly defined in terms of positivity and negativity by ELISA. This ELISA is suited for the rapid laboratory confirmation of AHS and should be considered as a replacement for the traditional AGID test.

UI	MeSH Term	Description	Entries
D010527	Perissodactyla	An order of ungulates having an odd number of toes, including the horse, tapir, and rhinoceros. (Dorland, 27th ed)	Perissodactylas
D011237	Predictive Value of Tests	In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test.	Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D012087	Reoviridae	A family of unenveloped RNA viruses with cubic symmetry. The twelve genera include ORTHOREOVIRUS; ORBIVIRUS; COLTIVIRUS; ROTAVIRUS; Aquareovirus, Cypovirus, Phytoreovirus, Fijivirus, Seadornavirus, Idnoreovirus, Mycoreovirus, and Oryzavirus.	Aquareovirus,Cypovirus,Cytoplasmic Polyhedrosis Viruses,Fijivirus,Idnoreovirus,Mycoreovirus,Oryzavirus,Phytoreovirus,Reoviruses, Aquatic,Respiratory Enteric Orphan Viruses,Seadornavirus,Aquareoviruses,Aquatic Reovirus,Aquatic Reoviruses,Cypoviruses,Cytoplasmic Polyhedrosis Virus,Fijiviruses,Idnoreoviruses,Mycoreoviruses,Oryzaviruses,Phytoreoviruses,Polyhedrosis Virus, Cytoplasmic,Polyhedrosis Viruses, Cytoplasmic,Reovirus, Aquatic,Seadornaviruses
D003429	Cross Reactions	Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.	Cross Reaction,Reaction, Cross,Reactions, Cross
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005779	Immunodiffusion	Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.	Gel Diffusion Tests,Diffusion Test, Gel,Diffusion Tests, Gel,Gel Diffusion Test,Immunodiffusions,Test, Gel Diffusion,Tests, Gel Diffusion
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006736	Horses	Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.	Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D000355	African Horse Sickness	An insect-borne reovirus infection of horses, mules and donkeys in Africa and the Middle East; characterized by pulmonary edema, cardiac involvement, and edema of the head and neck.	Equine Plague,African Horsesickness,African Horse Sicknesses,African Horsesicknesses,Equine Plagues,Horse Sickness, African,Horse Sicknesses, African,Horsesickness, African,Horsesicknesses, African,Plague, Equine,Plagues, Equine,Sickness, African Horse,Sicknesses, African Horse
D000356	African Horse Sickness Virus	A species of ORBIVIRUS that causes disease in horses, mules, and donkeys. Via its principal vector CULICOIDES, it can also infect dogs, elephants, camels, cattle, sheep, goats, and, in special circumstances, humans.

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