BIOMAT Database Logo BIOMAT Database Logo

Differential distribution of subsets of myofibrillar proteins in cardiac nonstriated and striated myofibrils. 1990

T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Department of Anatomy, School of Medicine, University of Pennsylvania, Philadelphia 19104.

Cultured cardiac myocytes were stained with antibodies to sarcomeric alpha-actinin, troponin-I, alpha-actin, myosin heavy chain (MHC), titin, myomesin, C-protein, and vinculin. Attention was focused on the distribution of these proteins with respect to nonstriated myofibrils (NSMFs) and striated myofibrils (SMFs). In NSMFs, alpha-actinin is found as longitudinally aligned, irregular approximately 0.3-microns aggregates. Such aggregates are associated with alpha-actin, troponin-I, and titin. These I-Z-I-like complexes are also found as ectopic patches outside the domain of myofibrils in close apposition to the ventral surface of the cell. MHC is found outside of SMFs in the form of discrete fibrils. The temporal-spatial distribution and accumulation of the MHC-fibrils with respect to the I-Z-I-like complexes varies greatly along the length of the NSMFs. There are numerous instances of I-Z-I-like complexes without associated MHC-fibrils, and also cases of MHC-fibrils located many microns from I-Z-I-like complexes. The transition between the terminal approximately 1.7-microns sarcomere of any given SMF and its distal NSMF-tip is abrupt and is marked by a characteristic narrow alpha-actinin Z-band and vinculin positive adhesion plaque. A titin antibody T20, which localizes to an epitope at the Z-band in SMFs, precisely costains the 0.3-microns alpha-actinin aggregates in ectopic patches and NSMFs. Another titin antibody T1, which in SMFs localizes to an epitope at the A-I junction, typically does not stain ectopic patches and NSMFs. Where detectable, the T1-positive material is adjacent to rather than part of the 0.3-microns alpha-actinin aggregates. Myomesin and C-protein are found only in their characteristic sarcomeric locations (even in just perceptible SMFs). These A-band-associated proteins appear to be absent in ectopic patches and NSMFs.

UI MeSH Term Description Entries
D009124 Muscle Proteins The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN. Muscle Protein,Protein, Muscle,Proteins, Muscle
D009206 Myocardium The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow. Muscle, Cardiac,Muscle, Heart,Cardiac Muscle,Myocardia,Cardiac Muscles,Heart Muscle,Heart Muscles,Muscles, Cardiac,Muscles, Heart
D009210 Myofibrils The long cylindrical contractile organelles of STRIATED MUSCLE cells composed of ACTIN FILAMENTS; MYOSIN filaments; and other proteins organized in arrays of repeating units called SARCOMERES . Myofilaments,Myofibril,Myofilament
D011494 Protein Kinases A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein. Protein Kinase,Kinase, Protein,Kinases, Protein
D002352 Carrier Proteins Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes. Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002642 Chick Embryo The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching. Embryo, Chick,Chick Embryos,Embryos, Chick
D003598 Cytoskeletal Proteins Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible. Proteins, Cytoskeletal
D005347 Fibroblasts Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. Fibroblast
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein

Related Publications

T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Degradation of rat cardiac myofibrils and myofibrillar proteins by a myosin-cleaving protease.
August 1979, Journal of biochemistry,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Elongation of cross-striated myofibrils.
May 1956, Biochimica et biophysica acta,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Phosphorylation of cardiac myofibrillar proteins.
January 1976, Recent advances in studies on cardiac structure and metabolism,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Human striated muscle myofibrils and actomyosin.
December 1967, Neurology,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Differential contribution of cardiac sarcomeric proteins in the myofibrillar force response to stretch.
October 2008, Pflugers Archiv : European journal of physiology,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Assembly and Maintenance of Myofibrils in Striated Muscle.
January 2017, Handbook of experimental pharmacology,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
The fine structure of striated myofibrils.
April 1954, The Journal of pathology and bacteriology,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Thermal denaturation of myofibrillar proteins of striated and smooth adductor muscles of scallop (Zygochlamys patagonica). A differential scanning calorimetric study.
February 2002, Journal of agricultural and food chemistry,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
The preparation and properties of mammalian striated myofibrils.
December 1948, The Journal of laboratory and clinical medicine,
T Schultheiss, and Z X Lin, and M H Lu, and J Murray, and D A Fischman, and K Weber, and T Masaki, and M Imamura, and H Holtzer
Calcium-sensitivity modulation of cardiac myofibrillar proteins.
January 1989, Journal of cardiovascular pharmacology,
Copied contents to your clipboard!