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The broad-host-range plasmid, pRW2, is a derivative of pRK 2501 carrying the Escherichia coli lac operon without a promoter, downstream of a polylinker sequence. We have cloned a number of DNA fragments carrying promoters into this plasmid and measured promoter activity in both E. coli and Pseudomonas aeruginosa. Promoters carrying consensus -10 and -35 sequences were active in both backgrounds and the dependence of activity on the nucleotide sequence of the 35 region was the same in both cases. We also measured the activity of two promoters at which transcription in E. coli was totally dependent on the E. coli activators CRP and FNR: both promoters were found to be active in P. aeruginosa.
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
December 1988,
Gene,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
May 1987,
Plasmid,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
May 1990,
Gene,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
August 1982,
Nucleic acids research,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
August 1982,
Nature,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
February 2001,
FEMS microbiology letters,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
April 1987,
Biotechnology and bioengineering,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
July 1992,
Molecular microbiology,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
February 1998,
Applied and environmental microbiology,
J Lodge, and
R Williams, and
A Bell, and
B Chan, and
S Busby
December 1982,
Journal of molecular biology,
