Biomaterial Database

Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria. 2011

Qian Chen, and Jinren Ni

Key Laboratory for Urban Habitat Environmental Science and Technology, School of Environment and Energy, Peking University Shenzhen Graduate School, 518055, Shenzhen, China.

Three novel strains capable of heterotrophic nitrification-aerobic denitrification were isolated from the landfill leachate treatment system. Based on their phenotypic and phylogenetic characteristics, the isolates were identified as Agrobacterium sp. LAD9, Achromobacter sp. GAD3 and Comamonas sp. GAD4, respectively. Batch tests were carried out to evaluate the growth and the ammonia removal patterns. The maximum growth rates as determined from the growth curve were 0.286, 0.228, and 0.433 h(-1) for LAD9, GAD3 and GAD4, respectively. The maximum aerobic nitrification-denitrification rate was achieved by the strain GAD4 of 0.381 mmol/l h, followed by LAD9 of 0.374 mmol/l h and GAD3 of 0.346 mmol/l h. Moreover, hydroxylamine oxidase and periplasmic nitrate reductase were successfully expressed in all the isolates. The relationship between the enzyme activities and the aerobic nitrification-denitrification rates revealed that hydroxylamine oxidation may be the rate-limiting step in the heterotrophic nitrification-aerobic denitrification process. The study results are of great significance to the wastewater treatment systems where simultaneous removal of carbon and nitrogen is desired.

UI	MeSH Term	Description	Entries
D010802	Phylogeny	The relationships of groups of organisms as reflected by their genetic makeup.	Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D000641	Ammonia	A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
D001420	Bacteria, Aerobic	Bacteria which require oxygen in order to grow and survive.	Aerobic Bacteria
D014865	Waste Disposal, Fluid	The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.	Disposal, Fluid Waste,Disposals, Fluid Waste,Fluid Waste Disposal,Fluid Waste Disposals,Waste Disposals, Fluid
D042402	Achromobacter	A genus of gram-negative, strictly aerobic, non-spore forming rods. Soil and water are regarded as the natural habitat. They are sometimes isolated from a hospital environment and humans.
D052836	Heterotrophic Processes	The processes by which organisms utilize organic substances as their nutrient sources. Contrasts with AUTOTROPHIC PROCESSES which make use of simple inorganic substances as the nutrient supply source. Heterotrophs can be either chemoheterotrophs (or chemoorganotrophs) which also require organic substances such as glucose for their primary metabolic energy requirements, or photoheterotrophs (or photoorganotrophs) which derive their primary energy requirements from light. Depending on environmental conditions some organisms can switch between different nutritional modes (AUTOTROPHY; heterotrophy; chemotrophy; or PHOTOTROPHY) to utilize different sources to meet their nutrients and energy requirements.	Heterotrophic Growth,Heterotrophic Process,Heterotrophy,Growth, Heterotrophic,Process, Heterotrophic,Processes, Heterotrophic
D058440	Denitrification	Nitrate reduction process generally mediated by anaerobic bacteria by which nitrogen available to plants is converted to a gaseous form and lost from the soil or water column. It is a part of the nitrogen cycle.	Denitrifications
D058465	Nitrification	A process facilitated by specialized bacteria involving the oxidations of ammonium to nitrite and nitrite to nitrate.	Aerobic Ammonia Oxidation,Nitrite Oxidation,Aerobic Ammonia Oxidations,Ammonia Oxidation, Aerobic,Nitrifications,Nitrite Oxidations,Oxidation, Aerobic Ammonia,Oxidation, Nitrite
D060054	Agrobacterium	A genus of gram negative, aerobic, rod-shaped bacteria found in soil, plants, and marine mud.
D019149	Bioreactors	Tools or devices for generating products using the synthetic or chemical conversion capacity of a biological system. They can be classical fermentors, cell culture perfusion systems, or enzyme bioreactors. For production of proteins or enzymes, recombinant microorganisms such as bacteria, mammalian cells, or insect or plant cells are usually chosen.	Fermentors,Bioreactor,Fermentor