-transparent.png){kind=logo}
High resolution mapping and positional cloning of ENU-induced mutations in the Rw region of mouse chromosome 5. 2010
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
BACKGROUND Forward genetic screens in mice provide an unbiased means to identify genes and other functional genetic elements in the genome. Previously, a large scale ENU mutagenesis screen was conducted to query the functional content of a ~50 Mb region of the mouse genome on proximal Chr 5. The majority of phenotypic mutants recovered were embryonic lethals. RESULTS We report the high resolution genetic mapping, complementation analyses, and positional cloning of mutations in the target region. The collection of identified alleles include several with known or presumed functions for which no mutant models have been reported (Tbc1d14, Nol14, Tyms, Cad, Fbxl5, Haus3), and mutations in genes we or others previously reported (Tapt1, Rest, Ugdh, Paxip1, Hmx1, Otoe, Nsun7). We also confirmed the causative nature of a homeotic mutation with a targeted allele, mapped a lethal mutation to a large gene desert, and localized a spermiogenesis mutation to a region in which no annotated genes have coding mutations. The mutation in Tbc1d14 provides the first implication of a critical developmental role for RAB-GAP-mediated protein transport in early embryogenesis. CONCLUSIONS This collection of alleles contributes to the goal of assigning biological functions to all known genes, as well as identifying novel functional elements that would be missed by reverse genetic approaches.
Related Publications
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
July 1995,
Genomics,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
July 1993,
Genomics,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
March 1996,
Genome research,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
March 1999,
Mammalian genome : official journal of the International Mammalian Genome Society,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
July 1994,
Proceedings of the National Academy of Sciences of the United States of America,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
December 1997,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
July 1998,
Mammalian genome : official journal of the International Mammalian Genome Society,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
August 2005,
Mammalian genome : official journal of the International Mammalian Genome Society,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
September 2004,
Mammalian genome : official journal of the International Mammalian Genome Society,
Yung-Hao Ching, and
Robert J Munroe, and
Jennifer L Moran, and
Anna K Barker, and
Evan Mauceli, and
Tim Fennell, and
Frederica Dipalma, and
Kerstin Lindblad-Toh, and
Lindsay M Abcunas, and
Joyanna F Gilmour, and
Tanya P Harris, and
Susan L Kloet, and
Yunhai Luo, and
John L McElwee, and
Weipeng Mu, and
Hyo K Park, and
David L Rogal, and
Kerry J Schimenti, and
Lishuang Shen, and
Mami Shindo, and
James Y Shou, and
Erin K Stenson, and
Patrick J Stover, and
John C Schimenti
October 1998,
Mammalian genome : official journal of the International Mammalian Genome Society,
