We investigated the on-farm potential of common farm invertebrates to transmit porcine circovirus genotype 2 (PCV2) and other non-enveloped viruses. In 2007 (pre-PCV2 vaccination) and 2008 (post-PCV2 vaccination), invertebrate communities were trap-collected (8 trap-dates per year), counted and sorted into genus and species groups on 5 farm study sites within England. Total DNA was extracted from feces of representational cross-sections of pigs on each farm in each year and also from intact samples of Diptera flies (ca. 20 flies per trap) and dissected viscera of any cockroaches (ca. 5 per trap). Each DNA sample was tested for the presence of PCV2 DNA by separate PCRs for ORF1 and ORF2. Positive samples were sub-typed via DNA sequencing of PCR products. The pig-associated Diptera fly community was dominated by Musca domestica (house fly) in both years on all 5 farms; numerous Blatta orientalis cockroaches were only noted on 1 farm throughout. Specific PCV2b DNA elements were routinely detected (25-60% of samples) in weaner/nursery pig feces in 2007, but not in other age groups. Musca collected on 4 of the 5 farms in 2007 was also positive for PCV2b DNA elements. Comparison of ORF2 sequences indicated that ORF2 sequences indicating PCV2b genotype were identical in pigs and flies. Minor changes were noted in ORF1 sequences from different samples. Flies collected in the weaner/nursery area were most likely to be positive (22-50% of fly-trap samples). DNA extracted from all cockroaches (2007 and 2008) and all flies and pig feces in 2008 were also negative throughout. We suggest that Musca flies have the most likely on-farm potential to carry and transmit PCV2b due to their life cycle incorporating stages in close association with pigs and their habitat. Vaccination appeared to reduce environmental load of PCV2b.