A method for quantitative analysis of inert gases in liquids by gas chromatography is described. The technique comprises an equilibration system for partial extraction of dissolved gases from liquids and a gas chromatograph equipped with helium ionization detector. The method is particularly devised for determination of solubility coefficients of gases in liquid materials, e.g. water, aqueous solutions, blood, tissue homogenates, and for simultaneous measurement of several inert gases dissolved in liquids. Coefficients of variation for serial analyses of individual samples were less than or equal to 3%. The method is applicable to studies of blood-tissue transfer of respiratory and inert gases.