-transparent.png){kind=logo}
A new method (type B) for isolation of canine islets was developed, utilizing intraductal collagenase perfusion, stationary digestion, filtration and tissue separation by means of a dextran density gradient. The results of this technique were compared with those of a previously established method (type A). Islets were autotransplanted either via the splenic or the portal vein. Lasting normoglycemia was obtained in 5/8 intrasplenic type B transplants (63%), 6/8 intrahepatic type B transplants (75%), 5/6 intrasplenic type A transplants (83%) and 0/3 intrahepatic type A transplants. No difference was found in metabolic parameters 2 weeks after successful transplantation following type A or B islet isolation. Islet yield was higher with type A and purification better with type B isolation. Both techniques are relatively simple and inexpensive. Because of its higher purification rate and the success of intrahepatic islet transplantation method B has replaced method A as the routine procedure in our laboratory.
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
December 1996,
Progres en urologie : journal de l'Association francaise d'urologie et de la Societe francaise d'urologie,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
January 1994,
The Mount Sinai journal of medicine, New York,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
December 1994,
Transplantation proceedings,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
October 1994,
Clinical transplantation,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
January 1963,
Nordisk medicin,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
January 1992,
Wiener klinische Wochenschrift,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
April 1987,
Transplantation,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
October 2007,
Proceedings (Baylor University. Medical Center),
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
March 1990,
Surgery, gynecology & obstetrics,
J A van der Vliet, and
D B Kaufman, and
R M Meloche, and
L G van der Hem, and
M J Field, and
D E Sutherland
August 2004,
Presse medicale (Paris, France : 1983),
