Mouse telomerase reverse transcriptase (mTert) expression marks slowly cycling intestinal stem cells. 2011

Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
Division of Gastroenterology, Children's Hospital Boston, Boston, MA 02115, USA.

The intestinal epithelium is maintained by a population of rapidly cycling (Lgr5(+)) intestinal stem cells (ISCs). It has been postulated, however, that slowly cycling ISCs must also be present in the intestine to protect the genome from accumulating deleterious mutations and to allow for a response to tissue injury. Here, we identify a subpopulation of slowly cycling ISCs marked by mouse telomerase reverse transcriptase (mTert) expression that can give rise to Lgr5(+) cells. mTert-expressing cells distribute in a pattern along the crypt-villus axis similar to long-term label-retaining cells (LRCs) and are resistant to tissue injury. Lineage-tracing studies demonstrate that mTert(+) cells give rise to all differentiated intestinal cell types, persist long term, and contribute to the regenerative response following injury. Consistent with other highly regenerative tissues, our results demonstrate that a slowly cycling stem cell population exists within the intestine.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007413 Intestinal Mucosa Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI. Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D043562 Receptors, G-Protein-Coupled The largest family of cell surface receptors involved in SIGNAL TRANSDUCTION. They share a common structure and signal through HETEROTRIMERIC G-PROTEINS. G Protein Coupled Receptor,G-Protein-Coupled Receptor,G-Protein-Coupled Receptors,G Protein Coupled Receptors,Receptor, G-Protein-Coupled,Receptors, G Protein Coupled
D049452 Green Fluorescent Proteins Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH. Green Fluorescent Protein,Green-Fluorescent Protein,Green-Fluorescent Proteins,Fluorescent Protein, Green,Fluorescent Proteins, Green,Protein, Green Fluorescent,Protein, Green-Fluorescent,Proteins, Green Fluorescent,Proteins, Green-Fluorescent
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D019070 Cell Lineage The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo. Cell Lineages,Lineage, Cell,Lineages, Cell
D019098 Telomerase An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES. Telomerase Catalytic Subunit,Telomerase Reverse Transcriptase,Telomerase Reverse Transcriptase Catalytic Subunit,Catalytic Subunit, Telomerase,Reverse Transcriptase, Telomerase,Subunit, Telomerase Catalytic,Transcriptase, Telomerase Reverse

Related Publications

Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
March 2022, Stem cells (Dayton, Ohio),
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
January 2009, Disease models & mechanisms,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
July 2019, Scientific reports,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
January 2011, Stem cells and development,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
June 2011, Molecular and cellular biology,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
January 2018, Human cell,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
January 2020, Scientific reports,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
March 2011, Cell cycle (Georgetown, Tex.),
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
June 2017, Cell stem cell,
Robert K Montgomery, and Diana L Carlone, and Camilla A Richmond, and Loredana Farilla, and Mariette E G Kranendonk, and Daniel E Henderson, and Nana Yaa Baffour-Awuah, and Dana M Ambruzs, and Laura K Fogli, and Selma Algra, and David T Breault
April 1998, Oncogene,
Copied contents to your clipboard!