Biomaterial Database

Characterization of lysosomal enzymes from cultured cynomolgus monkey trabecular meshwork cells. 1990

S Jeng, and R N Weinreb, and A L Miller

Department of Ophthalmology, University of California, San Diego 92093.

The current study characterizes selected properties of lysosomal enzymes associated with cynomolgus monkey trabecular meshwork (MTM) cells. These proteins may participate in the turnover of macromolecules involved in regulating the aqueous outflow. Intracellular levels of lysosomal enzymes in MTM cells were similar to those found in cultured human fibroblasts. The presence of ammonium chloride increased the secretion rate of certain lysosomal enzymes from 47 to 122% of normal. Column chromatography of the secreted enzymes on the galactose-specific lectin Ricinus communis I demonstrated an increase in the number of accessible galactose residues on lysosomal enzymes secreted in the presence of ammonium chloride. The presence of mannose-6-phosphate receptors on the trabecular meshwork cells was demonstrated by the specific uptake of purified 125I-beta-D-glucosidase. This uptake represented 20% of that observed with cultured human fibroblasts and was inhibited only 50% by the presence of mannose-6-phosphate.

UI	MeSH Term	Description	Entries
D008247	Lysosomes	A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured or undergoes MEMBRANE FUSION. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed).	Autolysosome,Autolysosomes,Lysosome
D008252	Macaca fascicularis	A species of the genus MACACA which typically lives near the coast in tidal creeks and mangrove swamps primarily on the islands of the Malay peninsula.	Burmese Long-Tailed Macaque,Crab-Eating Monkey,Cynomolgus Monkey,M. f. aurea,M. fascicularis,Macaca fascicularis aurea,Monkey, Crab-Eating,Monkey, Cynomolgus,Crab-Eating Macaque,Burmese Long Tailed Macaque,Crab Eating Macaque,Crab Eating Monkey,Crab-Eating Macaques,Crab-Eating Monkeys,Cynomolgus Monkeys,Long-Tailed Macaque, Burmese,Macaque, Burmese Long-Tailed,Macaque, Crab-Eating,Monkey, Crab Eating
D008360	Mannosephosphates	Phosphoric acid esters of mannose.
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D002846	Chromatography, Affinity	A chromatographic technique that utilizes the ability of biological molecules, often ANTIBODIES, to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Chromatography, Bioaffinity,Immunochromatography,Affinity Chromatography,Bioaffinity Chromatography
D004705	Endocytosis	Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.	Endocytoses
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006026	Glycoside Hydrolases	Any member of the class of enzymes that catalyze the cleavage of the glycosidic linkage of glycosides and the addition of water to the resulting molecules.	Endoglycosidase,Exoglycosidase,Glycohydrolase,Glycosidase,Glycosidases,Glycoside Hydrolase,Endoglycosidases,Exoglycosidases,Glycohydrolases,Hydrolase, Glycoside,Hydrolases, Glycoside
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000643	Ammonium Chloride	An acidifying agent that has expectorant and diuretic effects. Also used in etching and batteries and as a flux in electroplating.	Sal Ammoniac,Ammoniac, Sal,Chloride, Ammonium

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