Biomaterial Database

Arachidonic acid metabolism in heat-shock treated human leucocytes. 1990

M Köller, and W König

Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, FRG.

Human neutrophil granulocyte fractions (PMN) and lymphocytes/monocytes/basophils (LMB) were stimulated with A23187 (7.3 microM), opsonized zymosan (1 mg) or FMLP (10(-5) M) after heat-shock treatment. We observed a temperature- (pretreatment over 40 degrees) and time-dependent (incubation periods longer than 20 min) suppression in the generation of LTB4, LTB4 metabolites and isomers, as well as LTC4 and 5-HETE. These effects were not reversed after the addition of exogenous arachidonic acid (AA;50 microM). In contrast, heat-shock treatment alone triggered platelets to generate 12-HETE. After 1 hr at 42 degrees, 135 +/- 24 ng of 12-HETE were generated from 1 x 10(8) cells. The 12-HETE generation was not dependent on extracellular Ca2+. Conversion of 14C-AA (2 nmol) revealed an enhanced metabolism of AA to 12-HETE by platelets after heat-shock treatment without exogenous Ca2+. PMN and LMB labelled with 35S-methionine led to heat-shock protein (HSP; 65,000, 83,000 MW) expression after heat-shock treatment at 42 degrees or in the presence of NDGA (1 x 10(-5) M) at 37 degrees. These results suggest a regulatory interaction between the generation of lipo-oxygenase products, cellular stress responses and the expression of HSP.

UI	MeSH Term	Description	Entries
D007962	Leukocytes	White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).	Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D001792	Blood Platelets	Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.	Platelets,Thrombocytes,Blood Platelet,Platelet,Platelet, Blood,Platelets, Blood,Thrombocyte
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D006360	Heat-Shock Proteins	Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.	Stress Protein,Stress Proteins,Heat-Shock Protein,Heat Shock Protein,Heat Shock Proteins,Protein, Stress
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006893	Hydroxyeicosatetraenoic Acids	Eicosatetraenoic acids substituted in any position by one or more hydroxy groups. They are important intermediates in a series of biosynthetic processes leading from arachidonic acid to a number of biologically active compounds such as prostaglandins, thromboxanes, and leukotrienes.	HETE,Acids, Hydroxyeicosatetraenoic
D001095	Arachidonic Acids		Eicosatetraenoic Acids,Acids, Arachidonic,Acids, Eicosatetraenoic
D015289	Leukotrienes	A family of biologically active compounds derived from arachidonic acid by oxidative metabolism through the 5-lipoxygenase pathway. They participate in host defense reactions and pathophysiological conditions such as immediate hypersensitivity and inflammation. They have potent actions on many essential organs and systems, including the cardiovascular, pulmonary, and central nervous system as well as the gastrointestinal tract and the immune system.	Leukotriene
D016718	Arachidonic Acid	An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.	(all-Z)-5,8,11,14-Eicosatetraenoic acid,Arachidonic Acid, (all-Z)-Isomer, 1-(14)C-Labeled,Arachidonic Acid, (all-Z)-isomer, 3H-Labeled,Arachidonic Acid, Ammonium Salt, (all-Z)-Isomer,Arachidonic Acid, Cerium Salt, (all-Z)-Isomer,Arachidonic Acid, Cesium Salt, (all-Z)-Isomer,Arachidonic Acid, Lithium Salt, (all-Z)-Isomer,Arachidonic Acid, Potassium Salt, (all-Z)-Isomer,Arachidonic Acid, Sodium Salt,Arachidonic Acid, Sodium Salt, (all-Z)-Isomer,Arachidonic Acid, Zinc Salt, (all-Z)-Isomer,Sodium Arachidonate,Vitamin F,Arachidonate, Sodium