[Rapid virus diagnosis by electrosyneresis (author's transl)]. 1978

A Gaspar, and E Edlinger

Electrosyneresis (counter-current immunoelectrophoresis) detected the presence of 7 coxsackieviruses and 1 poliovirus in 70 specimens of stools. Identical results were obtained by inoculation of mice and tissue cultures. First screening of specimens was undertaken with a human "standard" gammaglobulin; only specimens precipitating with this reagent contained viral antigens. Hence, especially in children, all negative stools could be excluded from subsequent tests with antiserum pools and monospecific antisera. In our experience, electrosyneresis is a specific method: every type of coxsackievirus and poliovirus types react only with the corresponding monospecific antisera. Electrosyneresis appears to be a sensitive procedure: viral antigens corresponding to 10(4) TCID50/ml can be dtected.

UI MeSH Term Description Entries
D007122 Immunoelectrophoresis A technique that combines protein electrophoresis and double immunodiffusion. In this procedure proteins are first separated by gel electrophoresis (usually agarose), then made visible by immunodiffusion of specific antibodies. A distinct elliptical precipitin arc results for each protein detectable by the antisera.
D007223 Infant A child between 1 and 23 months of age. Infants
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D003378 Counterimmunoelectrophoresis Immunoelectrophoresis in which immunoprecipitation occurs when antigen at the cathode is caused to migrate in an electric field through a suitable medium of diffusion against a stream of antibody migrating from the anode as a result of endosmotic flow. Electrosyneresis,Immunoelectroosmophoresis,Immunoelectrophoresis, Countercurrent,Immunoelectrophoresis, Crossover,Countercurrent Immunoelectrophoresis,Crossover Immunoelectrophoresis
D003937 Diagnosis, Differential Determination of which one of two or more diseases or conditions a patient is suffering from by systematically comparing and contrasting results of diagnostic measures. Diagnoses, Differential,Differential Diagnoses,Differential Diagnosis
D004769 Enterovirus Infections Diseases caused by ENTEROVIRUS. Infections, Enterovirus,Enterovirus Infection,Infection, Enterovirus
D004770 Enterovirus A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus". Coxsackie Viruses,Coxsackieviruses
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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