Biomaterial Database

Dental pulp stem cells from primary and permanent teeth: quality analysis. 2010

Shobha Tandon, and Rooposhi Saha, and Ramesh Rajendran, and Rashmi Nayak

Manipal College of Dental Sciences, Manipal, Karnataka, India. drtandons@yahoo.com

OBJECTIVE The objective of the study was to identify and isolate stem cells from healthy and inflamed dental pulp and characterize their differentiation potential into multiple lineages. METHODS Study was conducted in dental pulp tissues obtained from the children in the age range of 5-14 years. Tissue samples were collected from teeth indicated for pulp therapy and extractions for orthodontic purpose. Samples were processed in the laboratory including cell culture, isolation and differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow which is considered as a gold standard. RESULTS There was no statistically significant difference found in the expression of various surface markers between inflamed dental pulp and bone marrow. Healthy pulp from the primary teeth was not sufficient to use as a source for harvesting stem cells moreover the healthy tissue obtained from permanent teeth failed to show any results at all. CONCLUSIONS Inflamed pulp discarded during pulp therapy procedures is a potential source for harvesting adult stem cells.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010865	Pilot Projects	Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work.	Pilot Studies,Pilot Study,Pilot Project,Project, Pilot,Projects, Pilot,Studies, Pilot,Study, Pilot
D011671	Pulpitis	Inflammation of the DENTAL PULP, usually due to bacterial infection in dental caries, tooth fracture, or other conditions causing exposure of the pulp to bacterial invasion. Chemical irritants, thermal factors, hyperemic changes, and other factors may also cause pulpitis.	Inflammation, Endodontic,Endodontic Inflammation,Endodontic Inflammations,Inflammations, Endodontic,Pulpitides
D001854	Bone Marrow Cells	Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.	Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002454	Cell Differentiation	Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.	Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002648	Child	A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL.	Children
D002675	Child, Preschool	A child between the ages of 2 and 5.	Children, Preschool,Preschool Child,Preschool Children
D003782	Dental Pulp	A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)	Dental Pulps,Pulp, Dental,Pulps, Dental
D005260	Female		Females