Biomaterial Database

Long-term karyological study of RVP3 tumour cells grown in vitro. 1978

H Sainerová, and J Svoboda

The RVP3 cell line established from an in vivo passaged RVP3 tumour, which originally arose in RSV-injected mice, was analyzed at different passage levels and characterized virologically and cytogenetically. The RVP3 cell line is characterized by the presence of 2 to 5 medium-sized to large metacentric chromosomes, the majority of the mitoses contain about 1 subtelocentric chromosome, and in addition, every mitosis contains several chromosomal structures corresponding to the group called m-ms that comprises both minute chromatin bodies and microchromosomes. In three monocellular clones derived from the RVP3 cell line with a similar karyological picture it was established that the number of m-ms increased proportionally to the total number of chromosomes in individual metaphases. It was verified that: the RVP3 cell population does not contain any transmissible agent producing chromosomal changes in mouse cells; RVP3 cells are highly malignant; no RSV could be rescued from the clone tested. The significance of m-ms is discussed.

UI	MeSH Term	Description	Entries
D007621	Karyotyping	Mapping of the KARYOTYPE of a cell.	Karyotype Analysis Methods,Analysis Method, Karyotype,Analysis Methods, Karyotype,Karyotype Analysis Method,Karyotypings,Method, Karyotype Analysis,Methods, Karyotype Analysis
D011831	Radiation Genetics	A subdiscipline of genetics that studies RADIATION EFFECTS on the components and processes of biological inheritance.	Genetics, Radiation
D002460	Cell Line	Established cell cultures that have the potential to propagate indefinitely.	Cell Lines,Line, Cell,Lines, Cell
D002999	Clone Cells	A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)	Clones,Cell, Clone,Cells, Clone,Clone,Clone Cell
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001357	Sarcoma, Avian	Connective tissue tumors, affecting primarily fowl, that are usually caused by avian sarcoma viruses.	Avian Sarcoma,Rous Sarcoma,Sarcoma, Rous,Avian Sarcomas,Sarcomas, Avian
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus