BIOMAT Database Logo BIOMAT Database Logo

Orderly microaggregates of G-/C-rich oligonucleotides associated with spermine. 2011

Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Key Laboratory for Green Chemical Technology MOE, Tianjin University, Tianjin 300072, People's Republic of China.

Spermine-induced orderly assembling properties of G-/C-rich oligonucleotides are investigated in dilute and crowding conditions. The first time we report that the parallel G-quadruplexes is preferential to condense into anisotropic microaggregates in the presence of spermine, whereas the hybrid-type and the antiparallel G-quadruplexes have no significant interactions with spermine; and spermine can induce the condensation of i-motif C-rich oligonucleotides other than the random coiled C-rich strands. Moreover, the condensation of C-rich oligonucleotides can be reversibly regulated by pH and temperature. G-/C-rich oligonucleotides exhibit the cholesteric liquid crystalline phase at low strand concentration in the presence of spermine under crowding conditions. The results illuminate that the parallel G-quadruplex and i-motifs are probably necessity conformations for G-/C-rich oligonucleotides that involved in the regulation of chromosome organization in living cells.

UI MeSH Term Description Entries
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D009841 Oligonucleotides Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed) Oligonucleotide
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D001482 Base Composition The relative amounts of the PURINES and PYRIMIDINES in a nucleic acid. Base Ratio,G+C Composition,Guanine + Cytosine Composition,G+C Content,GC Composition,GC Content,Guanine + Cytosine Content,Base Compositions,Base Ratios,Composition, Base,Composition, G+C,Composition, GC,Compositions, Base,Compositions, G+C,Compositions, GC,Content, G+C,Content, GC,Contents, G+C,Contents, GC,G+C Compositions,G+C Contents,GC Compositions,GC Contents,Ratio, Base,Ratios, Base
D013096 Spermine A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.
D013696 Temperature The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms. Temperatures
D044367 Phase Transition A change of a substance from one form or state to another. Gas-Liquid-Solid Phase Transitions,Sol-Gel Phase Transition,Gas Liquid Solid Phase Transitions,Gas-Liquid-Solid Phase Transition,Phase Transition, Gas-Liquid-Solid,Phase Transition, Sol-Gel,Phase Transitions,Phase Transitions, Gas-Liquid-Solid,Phase Transitions, Sol-Gel,Sol Gel Phase Transition,Sol-Gel Phase Transitions,Transition, Gas-Liquid-Solid Phase,Transition, Sol-Gel Phase,Transitions, Gas-Liquid-Solid Phase,Transitions, Sol-Gel Phase
D054856 G-Quadruplexes Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15) DNA G-Quadruplexes,DNA, Quadruplex,G-Quadruplexes, DNA,G-Quadruplexes, RNA,Guanine-Quadruplexes,Guanine-Quartets,Guanine-Tetrads,Quadruplex DNA,RNA, G-Quadruplexes,Tetraplex DNA,DNA G Quadruplexes,DNA, Tetraplex,G Quadruplexes,G Quadruplexes, DNA,G Quadruplexes, RNA,G-Quadruplexes RNA,G-Quadruplexes RNAs,Guanine Quadruplexes,Guanine Quartets,Guanine Tetrads,Guanine-Quartet,Guanine-Tetrad,RNA G-Quadruplexes,RNA, G Quadruplexes,RNAs, G-Quadruplexes

Related Publications

Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
G-rich oligonucleotides for cancer treatment.
January 2009, Methods in molecular biology (Clifton, N.J.),
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Antiproliferative activity of G-rich oligonucleotides correlates with protein binding.
September 1999, The Journal of biological chemistry,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
[Inhibition of leukemia cell proliferation by G-rich oligonucleotides].
February 2010, Zhongguo shi yan xue ye xue za zhi,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Use of short oligonucleotides to screen cosmid libraries for clones containing G/C-rich sequences.
June 1990, DNA and cell biology,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Synthesis of poly d(G-C) oligonucleotides.
January 2006, Nucleosides, nucleotides & nucleic acids,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Synthesis and biophysical characterization of G-rich oligonucleotides conjugated with sugar-phosphate tails.
January 2007, Nucleosides, nucleotides & nucleic acids,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Polymerase ribozyme efficiency increased by G/T-rich DNA oligonucleotides.
July 2011, RNA (New York, N.Y.),
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Nucleophosmin C-terminal leukemia-associated domain interacts with G-rich quadruplex forming DNA.
November 2010, The Journal of biological chemistry,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Short G-rich oligonucleotides as a potential therapeutic for Huntington's Disease.
October 2006, BMC neuroscience,
Yan Fu, and Xian Wang, and Jinli Zhang, and Ying Xiao, and Wei Li, and Jingkang Wang
Improved bioactivity of G-rich triplex-forming oligonucleotides containing modified guanine bases.
January 2014, Artificial DNA, PNA & XNA,
Copied contents to your clipboard!