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In Nb2 cell membranes, two guanine nucleotide-binding protein (G-protein) species (Mr 43.5 and 46.5 kD) were [32P]-ADP-ribosylated by cholera toxin, while a single protein (Mr 41.5 kD) was [32P]-ADP-ribosylated by pertussis toxin. Immunostaining indicated two immunoreactive prolactin (PRL) receptor moieties of 56 and 64 kD. Cross-linking with ethylene glyco bis[succinimidyl-succinate] (mol. length of 16.1 A) generated a high mol. wt., [32P]-ADP-ribosylated band of 140-160 kD which also showed immunoreactivity with antiserum to the PRL receptor. Other cross-linkers with shorter molecular lengths (8.6 - 11.4 A) were ineffective. These findings indicate that the Nb2 lactogen receptor is complexed with G-proteins and provide evidence for the role of G-proteins in mediating PRL-stimulated mitognesis in Nb2 cells.
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C K Too, and
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C K Too, and
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