Biomaterial Database

A combustion method for the simultaneous determination of 3H, 14C, and 35S in triply labeled organic samples by liquid scintillation counting. 1990

K Saito, and H Miyatake, and N Kurihara

Radioisotope Research Center, Kyoto University, Japan.

The organic samples triply labeled with 3H, 14C, and 35S were combusted in a flow-type combustion flask under a continuous oxygen flow. The oxygen gas containing the combustion products, which were mainly 3H2O, 14CO2, and 35SO2, was introduced into successive 0.5 M H2O2 and Oxisorb (2-methoxyethylamine). The 35SO2 and 3H2O that had been partially adsorbed onto the inner wall of the combustion flask were recovered by rinsing the wall with an H2O2 solution. 3H and 35S were quantitatively recovered from the H2O2 solutions. 14C was not found in the H2O2 solutions and was recovered from Oxisorb quantitatively. The amounts of 3H, 35S, and 14C were determined by measuring the radioactivity of the H2O2 solutions and of Oxisorb with a liquid scintillation counter. Thus the separate determination of 14C and 35S in a sample was achieved. Through this combustion method, the materials including fresh animal tissues up to ca. 300 mg were conveniently combusted with quantitative recovery of 3H, 14C, and 35S.

UI	MeSH Term	Description	Entries
D008954	Models, Biological	Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.	Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010100	Oxygen	An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.	Dioxygen,Oxygen-16,Oxygen 16
D002245	Carbon Dioxide	A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.	Carbonic Anhydride,Anhydride, Carbonic,Dioxide, Carbon
D002250	Carbon Radioisotopes	Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.	Radioisotopes, Carbon
D006861	Hydrogen Peroxide	A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.	Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D012588	Scintillation Counting	Detection and counting of scintillations produced in a fluorescent material by ionizing radiation.	Scintillation Counters,Counter, Scintillation,Counters, Scintillation,Counting, Scintillation,Scintillation Counter
D012996	Solutions	The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)	Solution
D013431	Sulfates	Inorganic salts of sulfuric acid.	Sulfate,Sulfates, Inorganic,Inorganic Sulfates
D013455	Sulfur	An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.	Sulfur-16,Sulfur 16
D013462	Sulfur Radioisotopes	Unstable isotopes of sulfur that decay or disintegrate spontaneously emitting radiation. S 29-31, 35, 37, and 38 are radioactive sulfur isotopes.	Radioisotopes, Sulfur