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alpha-Latrotoxin, the major toxin of black widow spider venom, was suggested to bind to the specific receptor on the membrane of presynaptic cells and to activate a nonselective cation channel. The aim of this investigation was to express the receptor to alpha-latrotoxin in the membrane of Xenopus laevis oocytes. Responses to alpha-latrotoxin were studied using a double microelectrode voltage-clamp technique on X. laevis oocytes previously injected with poly(A+)-RNA from rat brain. alpha-Latrotoxin (10 nM) was shown to induce a slow activating reversible inward membrane current at a clamp potential of -60 mV. A second application of alpha-latrotoxin immediately after washing out induced a smaller response. Reversal potential of this current was near to 0 mV; it hardly changed in low Cl- external solution. Response to alpha-latrotoxin did not depend significantly on the variation of Ca2+ concentration in external solution. Ethyleneglycolbis(aminoethylether)tetra-acetate (EGTA) injection into oocytes did not decrease alpha-latrotoxin-induced current, but seemed to slow the kinetics of the response. Inorganic Ca-channel blocker Co2+ had no effect on alpha-latrotoxin response. These results indicate that alpha-latrotoxin-induced inward current flows mainly through cation nonspecific channel. A lectin concanavalin A irreversibly inhibited alpha-latrotoxin-evoked inward current. Many of these observations are similar to those reported for nerve cells after alpha-latrotoxin application. The data obtained suggest that functional receptor to alpha-latrotoxin can be successively expressed in the membrane of Xenopus oocytes providing future search of DNA encoding receptor subunits and study of receptor structure-function relationship.
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
June 1990,
Brain research. Molecular brain research,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
October 1987,
The American journal of physiology,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
December 1988,
Neuroscience research,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
December 1988,
Proceedings of the National Academy of Sciences of the United States of America,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
January 1988,
Neirofiziologiia = Neurophysiology,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
September 1992,
Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
October 1991,
Proceedings of the National Academy of Sciences of the United States of America,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
January 1991,
Methods in cell biology,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
March 1987,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
A K Filippov, and
E M Kobrinsky, and
G P Tsurupa, and
V N Pashkov, and
E V Grishin
April 2002,
Molecular pharmacology,
