Biomaterial Database

Influence of carbohydrates on the isolation of lactic acid bacteria. 2011

A Endo, and Y Futagawa-Endo, and L M T Dicks

Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa Functional Foods Forum, University of Turku, Turku, Finland.

OBJECTIVE To determine the influence of carbohydrates on enrichment isolation of lactic acid bacteria from different niches. RESULTS Lactic acid bacteria in three traditional fermented products in southern Africa (amasi, mahewu and tshwala) and in three fresh samples (two flowers and a fruit) were enrichment cultured in media supplemented with 13 different carbohydrates. Diversity of lactic acid bacteria was determined by PCR-denaturing-gradient gel electrophoresis. Carbohydrates used in enrichment media had a big impact on the isolation of lactic acid bacteria from fermented products. Depending on the carbohydrates tested, the number of species detected ranged from one to four in amasi, one to five in mahewu and one to three in tshwala. Fructose and mannitol selected for relatively higher numbers of lactic acid bacteria in fermented products. Specific relationships between substrates and lactic acid bacteria have been noted. On the other hand, small influences were found among carbohydrates tested in flowers and fruit. CONCLUSIONS Carbohydrates have a big impact on the isolation of a variety of lactic acid bacteria in fermented food. CONCLUSIONS This is the first study that reports the influence of carbohydrates on the enrichment of lactic acid bacteria.

UI	MeSH Term	Description	Entries
D007778	Lactobacillus	A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Lactobacillus species are homofermentative and ferment a broad spectrum of carbohydrates often host-adapted but do not ferment PENTOSES. Most members were previously assigned to the Lactobacillus delbrueckii group. Pathogenicity from this genus is rare.
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D005285	Fermentation	Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.	Fermentations
D005516	Food Microbiology	The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.	Microbiology, Food
D016133	Polymerase Chain Reaction	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.	Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D050260	Carbohydrate Metabolism	Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.	Metabolism, Carbohydrate
D058645	Denaturing Gradient Gel Electrophoresis	Electrophoresis in which various denaturant gradients are used to induce nucleic acids to melt at various stages resulting in separation of molecules based on small sequence differences including SNPs. The denaturants used include heat, formamide, and urea.	Temperature Gradient Gel Electrophoresis
D035264	Flowers	The reproductive organs of plants.	Blooms, Plant,Blossoms,Pistil,Plant Style,Anther, Plant,Carpal, Plant,Carpals, Plant,Filament, Flower,Flower Filament,Ovary, Plant,Petals, Plant,Plant Anther,Plant Calyx,Plant Carpals,Plant Corolla,Plant Ovary,Plant Petals,Plant Sepals,Plant Stamen,Plant Stigma,Sepals, Plant,Stamen, Plant,Stigma, Plant,Style, Plant,Anthers, Plant,Bloom, Plant,Blossom,Calyx, Plant,Calyxs, Plant,Corolla, Plant,Corollas, Plant,Filaments, Flower,Flower,Flower Filaments,Ovaries, Plant,Petal, Plant,Pistils,Plant Anthers,Plant Bloom,Plant Blooms,Plant Calyxs,Plant Carpal,Plant Corollas,Plant Ovaries,Plant Petal,Plant Sepal,Plant Stamens,Plant Stigmas,Plant Styles,Sepal, Plant,Stamens, Plant,Stigmas, Plant,Styles, Plant