Head/tail interaction of vinculin influences cell mechanical behavior. 2011

Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
Center for Medical Physics and Technology, Biophysics Group, Friedrich-Alexander-University Erlangen-Nuremberg, Henkestrasse 91, 91052 Erlangen, Germany.

This study evaluates the influence of vinculin in closed conformation on the mechanical properties of cells. We demonstrate that MEFvin(-/-) cells transfected with the eGFP-vinculin mutant A50I (talin-binding-deficient-vinculin in a constitutively closed conformation) show 2-fold lower stiffness and focal adhesion density compared to MEFvin(+/+) and MEF(Rescue) cells. MEF(A50I) cells are as stiff as MEFvin(-/-) cells with similar focal adhesion density. Further, 2D traction microscopy indicates that MEF(A50I) and MEFvin(-/-) cells generate 3- to 4-fold less strain energy than MEFvin(+/+) and MEF(Rescue) cells. These results demonstrate that vinculin's mechano-coupling function is dependent on its conformational state.

UI MeSH Term Description Entries
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D016596 Vinculin A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10. alpha-Vinculin,beta-Vinculin,Isovinculin,alpha Vinculin,beta Vinculin
D049452 Green Fluorescent Proteins Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH. Green Fluorescent Protein,Green-Fluorescent Protein,Green-Fluorescent Proteins,Fluorescent Protein, Green,Fluorescent Proteins, Green,Protein, Green Fluorescent,Protein, Green-Fluorescent,Proteins, Green Fluorescent,Proteins, Green-Fluorescent
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D055595 Mechanical Phenomena The properties and processes of materials that affect their behavior under force. Mechanical Concepts,Mechanical Processes,Mechanical Phenomenon,Mechanical Process,Concept, Mechanical,Concepts, Mechanical,Mechanical Concept,Phenomena, Mechanical,Phenomenon, Mechanical,Process, Mechanical,Processes, Mechanical
D022001 Focal Adhesions An anchoring junction of the cell to a non-cellular substrate. It is composed of a specialized area of the plasma membrane where bundles of the ACTIN CYTOSKELETON terminate and attach to the transmembrane linkers, INTEGRINS, which in turn attach through their extracellular domains to EXTRACELLULAR MATRIX PROTEINS. Adhesion Plaques,Cell-Matrix Adherens Junctions,Focal Contacts,Adherens Junction, Cell-Matrix,Adherens Junctions, Cell-Matrix,Adhesion Plaque,Adhesion, Focal,Adhesions, Focal,Cell Matrix Adherens Junctions,Cell-Matrix Adherens Junction,Contact, Focal,Contacts, Focal,Focal Adhesion,Focal Contact,Junction, Cell-Matrix Adherens,Junctions, Cell-Matrix Adherens,Plaque, Adhesion,Plaques, Adhesion

Related Publications

Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
June 2016, Integrative biology : quantitative biosciences from nano to macro,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
December 2009, Biophysical journal,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
March 2010, Cell biology international,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
August 2008, Biochemical and biophysical research communications,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
April 2005, The Journal of biological chemistry,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
January 2022, Scientific reports,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
April 1994, The Journal of biological chemistry,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
January 1995, Nature,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
May 2024, Bio-medical materials and engineering,
Gerold Diez, and Vera Auernheimer, and Ben Fabry, and Wolfgang H Goldmann
February 2020, Angewandte Chemie (International ed. in English),
Copied contents to your clipboard!