Biomaterial Database

Temporal analysis of retinal function in IRBP peptide-induced experimental autoimmune uveoretinitis (EAU). 1990

J C Waldrep, and S Ramanadham, and J D Wood, and L A Donoso

Center for Biotechnology, Baylor College of Medicine, The Woodlands, TX 77381.

Experimental autoimmune uveoretinitis (EAU) induced by interphotoreceptor retinoid binding protein (IRBP) is a complex and multifaceted disease mediated by several different immunopathogenic processes. There is a temporal progression of the disease affecting the anterior and posterior segments of the eye, culminating with destruction of the photoreceptor cells. In the present study, we investigated the effects of IRBP peptide-induced EAU on different cell types within the retina. The Muller cells of the peripheral retina are damaged at the onset of EAU at day 9. There is induction of glial fibrillary acidic protein (GFAP) in the peripheral retinal Muller cells with concomitant reduction in the electroretinogram (ERG) b-wave. By day 12 of EAU, intensely GFAP-positive Muller cells are detected throughout the peripheral and central retina and with maximal diminution of the ERG b-wave. There is a lag in the diminution of the ERG a-wave, first detected at day 12 of EAU, and reaching its maximum at day 15. This change in the ERG is associated with destruction of the tips of the photoreceptor cells. These results indicate that during IRBP peptide-induced EAU, there is a positive correlation between the temporal changes in retinal histology, GFAP expression by Muller cells, and loss of retinal function.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011917	Rats, Inbred Lew	An inbred strain of rat that is used in BIOMEDICAL RESEARCH.	Rats, Inbred Lewis,Rats, Lew,Inbred Lew Rat,Inbred Lew Rats,Inbred Lewis Rats,Lew Rat,Lew Rat, Inbred,Lew Rats,Lew Rats, Inbred,Lewis Rats, Inbred,Rat, Inbred Lew,Rat, Lew
D012160	Retina	The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.	Ora Serrata
D004596	Electroretinography	Recording of electric potentials in the retina after stimulation by light.	Electroretinographies
D005136	Eye Proteins	PROTEINS derived from TISSUES of the EYE.	Proteins, Eye
D005260	Female		Females
D005904	Glial Fibrillary Acidic Protein	An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.	Glial Intermediate Filament Protein,Astroprotein,GFA-Protein,Glial Fibrillary Acid Protein,GFA Protein
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001327	Autoimmune Diseases	Disorders that are characterized by the production of antibodies that react with host tissues or immune effector cells that are autoreactive to endogenous peptides.	Autoimmune Disease,Disease, Autoimmune,Diseases, Autoimmune