-transparent.png){kind=logo}
The relationship between spectrotype and IgG subclass of autoantibodies to thyroglobulin (Tg) and thyroid peroxidase (TPO) has been investigated using sera from Hashimoto and Graves' patients. Isoelectric focussing (IEF) was carried out in gels over the range pI 3.5-9.5 followed by transblotting to nitrocellulose and probing the filters using 125I-labelled TPO and Tg. As has been shown previously for Tg antibodies, TPO antibody focussed over different pI values in different patients but the spectrotypes for individuals were constant over 2-5 years. Further, the pI values for TPO and Tg autoantibodies appeared to be related to IgG subclass, for example IgG1 Tg antibodies tended to focus nearer the cathode (pI 8.0-9.5) than IgG4 antibodies (pI 6.5-8.5) while antibodies of subclasses IgG1 + 2 + 4 produced spectrotypes covering a broad pI range (5.7-9.5). Consequently, it seems likely that the characteristic spectrotypes described by others for Tg autoantibodies, and those we now report for TPO antibodies, reflect the IgG subclass "fingerprints" which we suggest may be a measure of the ability of an individual to respond to different epitopes on these two thyroid antigens.
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
May 2011,
Autoimmunity,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
January 1986,
Monographs in allergy,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
June 2015,
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
November 1986,
Immunology letters,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
June 2010,
Nihon rinsho. Japanese journal of clinical medicine,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
January 1997,
Zhonghua Minguo xiao er ke yi xue hui za zhi [Journal]. Zhonghua Minguo xiao er ke yi xue hui,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
April 1992,
Clinical immunology and immunopathology,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
January 1986,
Clinical and experimental immunology,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
August 2022,
eLife,
N Fukuma, and
S M McLachlan, and
V B Petersen, and
K Beever, and
B R Smith
November 1999,
Nihon rinsho. Japanese journal of clinical medicine,
