-transparent.png){kind=logo}
In order to clone the human lymphotoxin (HuLT) gene, we practiced a concise and time-saving method: homologous recombination in vivo (1). By using the mouse lymphotoxin (MuLT) cDNA (1.3 kb) as a probe, we isolated the HuLT gene from a human genomic library which was constructed with cosmid pcos2EMBL as a vector. After linearization, the recombinant cosmid was partially digested with BamHI, EcoRI, PstI, and PvuII respectively, and either cos end was labelled by hybridization with radioactive oligos complementary to the cohesive end sequence (2). The physical map of HuLT gene was made by this method.
Related Publications
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
April 1996,
Biochemical and biophysical research communications,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
July 1992,
European journal of biochemistry,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
August 2002,
International journal of molecular medicine,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
December 1995,
Biochemical and biophysical research communications,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
November 1983,
Plasmid,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
April 1994,
Biochemical and biophysical research communications,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
July 1986,
The Journal of general virology,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
March 2001,
Biochimica et biophysica acta,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
March 1998,
Genomics,
L H Li, and
W Y Zhuang, and
J H Chai, and
C B Li, and
S Y Zhao
September 1983,
Scientia Sinica. Series B, Chemical, biological, agricultural, medical & earth sciences,
