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Human growth hormone releasing factor (hGRF) gene has been synthesized and cloned. The sequence of the synthetic hGRF gene, consisting of preferred codons for expression in E. coli, was designed with the aid of computer programs. Six segments with lengths ranging from 39 to 51 nucleotides were synthesized by solid-phase phosphoramidite method. The entire gene of 141 base pairs was constructed by enzymatic ligation of all synthetic segments and then cloned into plasmid pUC-19. The positive colonies were confirmed by the screening of ampicillin resistance, inactive beta-galactosidase, and analyzing by use of restriction enzymes and dot-blot hybridization. The cloned gene was sequenced by M13 dideoxynucleotide chain termination method and proven correct.
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
May 1988,
Bioorganicheskaia khimiia,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
January 1985,
Biochimie,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
January 1987,
Methods in enzymology,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
January 1991,
Chinese journal of biotechnology,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
January 1983,
Nature,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
April 1999,
The Journal of biological chemistry,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
January 1986,
Sheng li ke xue jin zhan [Progress in physiology],
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
October 1971,
Biochemical and biophysical research communications,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
September 1983,
Biochemical and biophysical research communications,
K Y Xiong, and
R Z Jin, and
K H Hu, and
D M Ding
July 1983,
Proceedings of the National Academy of Sciences of the United States of America,
