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The presence of bacteria in periapical lesions of teeth with necrotic pulp has been demonstrated by microbiological sampling of periapical lesions during endodontic surgery. The purpose of this study was to confirm the presence of Bacteroides intermedius in a periapical granuloma using an indirect immunofluorescence technique. Histologic sections of a periapical granuloma were incubated with a rabbit antiserum to B. intermedius. After incubation with secondary antibody (fluorescein-conjugated goat anti-rabbit IgG), an intense fluorescent staining was observed in areas of the tissue sections. Control tissue sections that were incubated without the antibody remained unstained. The results obtained with the indirect immunofluorescence technique supported our cultural findings that microorganisms, e.g. B. intermedius, were present in the tissue of the periapical granuloma.
F Barnett, and
R Stevens, and
L Tronstad
January 2006,
Sub-cellular biochemistry,
F Barnett, and
R Stevens, and
L Tronstad
January 1976,
Verhandlungen der Deutschen Gesellschaft fur Rheumatologie,
F Barnett, and
R Stevens, and
L Tronstad
November 1985,
Chinese medical journal,
F Barnett, and
R Stevens, and
L Tronstad
January 1979,
Medical microbiology and immunology,
F Barnett, and
R Stevens, and
L Tronstad
January 1971,
Dermatologische Monatschrift,
F Barnett, and
R Stevens, and
L Tronstad
January 1976,
Zeitschrift fur Rheumatologie,
F Barnett, and
R Stevens, and
L Tronstad
January 1998,
Methods in cell biology,
F Barnett, and
R Stevens, and
L Tronstad
December 1967,
The Journal of investigative dermatology,
F Barnett, and
R Stevens, and
L Tronstad
November 1974,
Zeitschrift fur die gesamte innere Medizin und ihre Grenzgebiete,
F Barnett, and
R Stevens, and
L Tronstad
January 1984,
The British journal of dermatology,
