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The ability to investigate the proteome of formalin-fixed, paraffin-embedded (FFPE) tissues can be considered a major recent achievement in the field of clinical proteomics. However, gel-based approaches to the investigation of FFPE tissue proteomes have lagged behind, mainly because of insufficient quality of full-length protein extracts. Here, the 2-D DIGE technology was investigated for applicability to FFPE proteins, for internal reproducibility among replicate FFPE extracts, and for comparability between FFPE and fresh-frozen tissue profiles. The 2-D DIGE patterns obtained upon labeling and electrophoresis of replicate FFPE tissue extracts were highly reproducible, with satisfactory resolution and complexity. Moreover, the implementation of DIGE enabled to highlight and characterize the consistent differences found in the FFPE profiles compared with fresh-frozen profiles, represented by an acidic shift, directly correlated to the protein pI value, and by a reduction in spot signal intensity, directly correlated to molecular weight and percentage of lysine residues. Being constantly and reproducibly present in all FFPE tissue extract replicates at similar extents, these modifications do not appear to hinder the comparative analysis of FFPE tissue extracts by 2-D DIGE, opening the way to its application for the differential proteomic investigation of archival tissue repositories.
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
April 2014,
The protein journal,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
April 2013,
Expert review of proteomics,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
January 2011,
Methods in molecular biology (Clifton, N.J.),
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
March 2009,
Clinics in laboratory medicine,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
May 1997,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
February 2002,
Forensic science international,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
January 1998,
Methods in molecular biology (Clifton, N.J.),
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
September 2008,
Histochemistry and cell biology,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
December 2006,
Fa yi xue za zhi,
Alessandro Tanca, and
Daniela Pagnozzi, and
Giovanni Falchi, and
Roberto Tonelli, and
Stefano Rocca, and
Tonina Roggio, and
Sergio Uzzau, and
Maria Filippa Addis
January 2010,
Acta pharmacologica Sinica,
