The objective of this study was to understand the perception of a multidisciplinary health team regarding the implementation of a quality management took at a teaching hospital. The setting of this qualitative study was the University Hospital at University of São Paulo (HU-USP). Participants were seven workers from the Committee for the Implementation of the 5S Program. Data collection was performed through semi-structured interviews and the statements were analyzed according to Janesick. Two categories emerged from the statements: The perception of the multidisciplinary team participating in the implementation of the 5S Program and Factors that affect the implementation of the 5S Program. The categories were interpreted following the Donabedian framework. This study permitted to understand the perception of the multidisciplinary team regarding the implementation of a quality management tool. It is believed that the present findings will contribute with evaluating the 5S Program at the HU-USP, and provide the necessary support for reorganizing activities at that hospital.