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Human term placental villi cultured "in vitro" were maintained with bloodstream forms of Trypanosoma cruzi during various periods of time. Two different concentrations of the parasite were employed. Controls contained no T. cruzi. The alkaline phosphatase activity was determined in placental villi by electron microscopy and its specific activity in the culture medium by biochemical methods. Results showed that the hemoflagellate produces a significant decrease in enzyme activity as shown by both ultracytochemical and specific activity studies and this activity was lower in cultures with high doses of parasites. The above results indicate that the reduction in enzyme activity coincides with the time of penetration and proliferation of T. cruzi in mammalian cells. These changes may represent an interaction between human trophoblast and T. cruzi.
R E Fretes, and
S P de Fabro
January 2005,
Placenta,
R E Fretes, and
S P de Fabro
February 2002,
Experimental and molecular pathology,
R E Fretes, and
S P de Fabro
January 2005,
Revista da Sociedade Brasileira de Medicina Tropical,
R E Fretes, and
S P de Fabro
January 1985,
Comparative biochemistry and physiology. B, Comparative biochemistry,
R E Fretes, and
S P de Fabro
October 1991,
Experimental parasitology,
R E Fretes, and
S P de Fabro
May 1978,
Infection and immunity,
R E Fretes, and
S P de Fabro
May 2009,
Experimental parasitology,
R E Fretes, and
S P de Fabro
January 1983,
Zeitschrift fur Parasitenkunde (Berlin, Germany),
R E Fretes, and
S P de Fabro
August 1981,
The Journal of parasitology,
R E Fretes, and
S P de Fabro
January 1977,
Revista do Instituto de Medicina Tropical de Sao Paulo,
