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Estimation of Free Thiols and Disulfide Bonds Using Ellman's Reagent. 2008

Richard J Simpson

INTRODUCTIONThis protocol describes a method for quantitating the amount of free thiols and disulfide bond linkages in proteins. It uses the sulfhydryl reagent 5,5'-dithiobis (2-nitrobenzoic acid), or DTNB, which is also referred to as Ellman's reagent.

UI MeSH Term Description Entries

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