-transparent.png){kind=logo}
To elucidate the possible mechanism(s) of vinblastine-induced premature ovarian failure, we studied the effect of vinblastine (VLB) on progesterone (P) and prostaglandin E (PGE) production by rat granulosa cells in vitro. Granulosa cells obtained from immature, pregnant mares' serum gonadotrophin-primed rats were incubated for 5 h in modified Medium 199 +/- LH (10 ng/mL) with varying concentrations of VLB (0.001 to 10.0 micrograms/mL) and cells plus media were assayed for total P and PGE. VLB reduced production of both basal and LH-stimulated P in granulosa cells, with VLB at a concentration of 0.1 micrograms/mL showing the first significant difference from control. This dose also suppressed basal and LH-stimulated PGE. Granulosa cell survival was similar for all groups. Thus VLB, an agent known to disrupt microtubular function, reduced granulosa cell production of both P and PGE. Our results suggest that at a concentration (0.1 micrograms/mL) lower than that routinely achieved during chemotherapy (0.3 micrograms/mL), VLB depresses rat granulosa cell function in vitro. This system appears to be a valid model for preliminary assessment of the cytotoxic effects of chemotherapy on an ovarian component.
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
January 2001,
Biological signals and receptors,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
November 1982,
Endocrinology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
February 1994,
European journal of endocrinology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
May 1998,
Endocrine research,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
January 1977,
Endocrinology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
January 1982,
Endocrinology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
October 1984,
The American journal of physiology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
June 1997,
Endocrinology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
April 1990,
Endocrinology,
N L Teaff, and
R T Savoy-Moore, and
M G Subramanian, and
K M Ataya
March 1994,
European journal of endocrinology,
