[Effects of acute lymphoblastic leukemia children bone marrow mesenchymal stem cells on drug resistance of K562/A02 cell line]. 2011

Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
Department of Pediatrics, Wendeng Central Hospital, Weihai 264400, Shandong Province, China.

The aim of study was to investigate the effect of acute lymphoblastic leukemia (ALL) children bone marrow mesenchymal stem cells (MSC) on resistance of K562/A02 cells and its mechanism. MSC obtained from bone marrow of AL children were cultured and identified. The co-culture of MSC and K562/A02 and the culture of K562/A02 cell suspension alone was performed, of which 2 kinds of cells were treated with same concentration of adriamycin (ADM), and the rate of apoptosis was detected by flow cytometry, bcl-2 and bax of K562/A02 were detected by RT-PCR, while mdr1 gene level was detected by FQ-PCR. The results indicated that the MSC separation and proliferation were viable and steady. The apoptosis rate of the K562/A02 cells co-cultured with MSC was 1.97 ± 0.11%, while apoptosis rate of the K562/A02 cells cultured alone was 8.38 ± 0.29%, there was significant difference (p < 0.05). As compared with the K562/A02 cells cultured alone, the bcl-2 gene expression in K562/A02 cells co-cultured with MSC obviously increased; ratio of bcl-2/bax was obviously enhanced. The mdr1 gene level in K562/A02 co-cultured with MSC was no statistical different from K562/A02 cultured alone (p > 0.05), which suggested that adhesion co-cultured with MSC did not induce mdr1 expression higher than the culture of suspension. It is concluded that the MSC of ALL children can escape the leukemia cells from proapoptotic effect of drugs, the resistance of K562/A02 to ADM may be involved in enhancement of bcl-2 gene expression of K562/A02 cells co-cultured with MSC, but not in relation to mdr1 gene in K562/A02 cells themselves.

UI MeSH Term Description Entries
D008297 Male Males
D001854 Bone Marrow Cells Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells. Bone Marrow Cell,Cell, Bone Marrow,Cells, Bone Marrow,Marrow Cell, Bone,Marrow Cells, Bone
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D004317 Doxorubicin Antineoplastic antibiotic obtained from Streptomyces peucetius. It is a hydroxy derivative of DAUNORUBICIN. Adriamycin,Adriablastin,Adriablastine,Adriblastin,Adriblastina,Adriblastine,Adrimedac,DOXO-cell,Doxolem,Doxorubicin Hexal,Doxorubicin Hydrochloride,Doxorubicin NC,Doxorubicina Ferrer Farm,Doxorubicina Funk,Doxorubicina Tedec,Doxorubicine Baxter,Doxotec,Farmiblastina,Myocet,Onkodox,Ribodoxo,Rubex,Urokit Doxo-cell,DOXO cell,Hydrochloride, Doxorubicin,Urokit Doxo cell
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015973 Gene Expression Regulation, Leukemic Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in leukemia. Leukemic Gene Expression Regulation,Regulation of Gene Expression, Leukemic,Regulation, Gene Expression, Leukemic
D051028 bcl-2-Associated X Protein A member of the Bcl-2 protein family and homologous partner of C-BCL-2 PROTO-ONCOGENE PROTEIN. It regulates the release of CYTOCHROME C and APOPTOSIS INDUCING FACTOR from the MITOCHONDRIA. Several isoforms of BCL2-associated X protein occur due to ALTERNATIVE SPLICING of the mRNA for this protein. Bax Protein,Bax-alpha Protein,Bax-omega Protein,Bax-sigma Protein,Bax Apoptosis Regulator Protein,Bax-beta Protein,Bax-delta Protein,bcl2-Associated X Protein,bcl2-Associated X Protein Isoform alpha,bcl2-Associated X Protein Isoform beta,bcl2-Associated X Protein Isoform delta,bcl2-Associated X Protein Isoform omega,bcl2-Associated X Protein Isoform sigma,Bax alpha Protein,Bax beta Protein,Bax delta Protein,Bax omega Protein,Bax sigma Protein,Protein, bcl-2-Associated X,X Protein, bcl-2-Associated,bcl 2 Associated X Protein,bcl2 Associated X Protein,bcl2 Associated X Protein Isoform alpha,bcl2 Associated X Protein Isoform beta,bcl2 Associated X Protein Isoform delta,bcl2 Associated X Protein Isoform omega,bcl2 Associated X Protein Isoform sigma
D054198 Precursor Cell Lymphoblastic Leukemia-Lymphoma A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias. Leukemia, Lymphoblastic,Leukemia, Lymphoid, Acute,Lymphoblastic Leukemia,Lymphoblastic Lymphoma,Lymphocytic Leukemia, Acute,Lymphoma, Lymphoblastic,ALL, Childhood,Acute Lymphoid Leukemia,Leukemia, Acute Lymphoblastic,Leukemia, Lymphoblastic, Acute,Leukemia, Lymphoblastic, Acute, L1,Leukemia, Lymphoblastic, Acute, L2,Leukemia, Lymphoblastic, Acute, Philadelphia-Positive,Leukemia, Lymphocytic, Acute,Leukemia, Lymphocytic, Acute, L1,Leukemia, Lymphocytic, Acute, L2,Lymphoblastic Leukemia, Acute,Lymphoblastic Leukemia, Acute, Adult,Lymphoblastic Leukemia, Acute, Childhood,Lymphoblastic Leukemia, Acute, L1,Lymphoblastic Leukemia, Acute, L2,Lymphocytic Leukemia, L1,Lymphocytic Leukemia, L2,Acute Lymphoblastic Leukemia,Acute Lymphocytic Leukemia,Childhood ALL,L1 Lymphocytic Leukemia,L2 Lymphocytic Leukemia,Leukemia, Acute Lymphocytic,Leukemia, Acute Lymphoid,Leukemia, L1 Lymphocytic,Leukemia, L2 Lymphocytic,Lymphoid Leukemia, Acute,Precursor Cell Lymphoblastic Leukemia Lymphoma

Related Publications

Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
June 2007, Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
October 2016, Zhongguo shi yan xue ye xue za zhi,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
November 2008, Ai zheng = Aizheng = Chinese journal of cancer,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
April 2024, International journal of molecular sciences,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
February 2009, Zhongguo shi yan xue ye xue za zhi,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
December 1997, Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
April 2009, Zhongguo shi yan xue ye xue za zhi,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
January 2014, Journal of B.U.ON. : official journal of the Balkan Union of Oncology,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
February 2010, Zhongguo shi yan xue ye xue za zhi,
Zhao-Xia Wang, and Zhi-Min Yang, and Ya-Wei Zou, and Min-Min Li, and Fu-Xiong Chen, and Guo-Yu Zhong, and Jing-Ming Guan, and Feng-Gui Wei, and Shang-Zhi Wu, and Zheng-Tao He, and Zi-Liang Wu
April 2024, Zhongguo shi yan xue ye xue za zhi,
Copied contents to your clipboard!