-transparent.png){kind=logo}
Sep-Pak C18 extraction of human plasma and radioimmunoassay using antibodies which recognize atrial natriuretic peptide (99-128) and the prohormone sequences 1-30 and 31-67 resulted in mean values from 20 normal subjects of 26.2 (+/- 9.2), 362 (+/- 173) and 368 (+/- 160) pg/ml, respectively. A high correlation coefficient between values obtained using antibodies recognizing prohormone sequences 1-30 and 31-67 was observed (R = 0.84). Extracted plasma immunoreactivity of 1-30 and 31-67 both eluted at 46% acetonitrile. In contrast, chromatographic elution of synthetic peptides 1-30 and 31-67 was observed at 48 and 39% acetonitrile, respectively. Data suggest that the radioimmunoassay of plasma using antibodies recognizing prohormone sequences 1-30 and 31-67 may represent the measurement of a unique larger amino-terminal peptide fragment containing antigenic sites recognized by both antisera.
Y M Chen, and
P A Whitson, and
N M Cintrón
November 1987,
Biochemical and biophysical research communications,
Y M Chen, and
P A Whitson, and
N M Cintrón
January 1990,
Peptides,
Y M Chen, and
P A Whitson, and
N M Cintrón
January 1988,
Biochemical and biophysical research communications,
Y M Chen, and
P A Whitson, and
N M Cintrón
January 1988,
Chronobiology international,
Y M Chen, and
P A Whitson, and
N M Cintrón
April 1989,
The American journal of the medical sciences,
Y M Chen, and
P A Whitson, and
N M Cintrón
February 1989,
American heart journal,
Y M Chen, and
P A Whitson, and
N M Cintrón
September 1994,
Clinical science (London, England : 1979),
Y M Chen, and
P A Whitson, and
N M Cintrón
August 1990,
The American journal of the medical sciences,
Y M Chen, and
P A Whitson, and
N M Cintrón
December 1989,
The American journal of the medical sciences,
Y M Chen, and
P A Whitson, and
N M Cintrón
January 1997,
The Journal of experimental biology,
