Biomaterial Database

An ungrouped plant kinesin accumulates at the preprophase band in a cell cycle-dependent manner. 2011

Jennelle L Malcos, and Richard J Cyr

Department of Biology, The Pennsylvania State University, 208 Mueller Laboratory, University Park, Pennsylvania, USA.

Past phylogenic studies have identified a plant-specific, ungrouped family of kinesins in which the motor domain does not group to one of the fourteen recognized families. Members of this family contain an N-terminal motor domain, a C-terminal armadillo repeat domain and a conserved destruction box (D-BOX) motif. This domain architecture is unique to plants and to a subset of protists. Further characterization of one representative member from Arabidopsis, Arabidopsis thaliana KINESIN ungrouped clade, gene A (AtKINUa), was completed to ascertain its functional role in plants. Fluorescence confocal microscopy revealed an accumulation of ATKINUA:GFP at the preprophase band (PPB) in a cell cycle-dependent manner in Arabidopsis epidermal cells and tobacco BY-2 cells. Fluorescence accumulation was highest during prophase and decreased after nuclear envelope breakdown. A conserved D-BOX motif was identified through alignment of AtKINU homologous sequences. Mutagenesis work with D-BOX revealed that conserved residues were necessary for the observed degradation pattern of ATKINUA:GFP, as well as the targeted accumulation at the PPB. Overall results suggest that AtKINUa is necessary for normal plant growth and/or development and is likely involved with PPB organization through microtubule association and specific cell cycle regulation. The D-BOX motif may function to bridge microtubule organization with changes that occur during progression through mitosis and may represent a novel regulatory motif in plant microtubule motor proteins.

UI	MeSH Term	Description	Entries
D008870	Microtubules	Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.	Microtubule
D008938	Mitosis	A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.	M Phase, Mitotic,Mitotic M Phase,M Phases, Mitotic,Mitoses,Mitotic M Phases,Phase, Mitotic M,Phases, Mitotic M
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010802	Phylogeny	The relationships of groups of organisms as reflected by their genetic makeup.	Community Phylogenetics,Molecular Phylogenetics,Phylogenetic Analyses,Phylogenetic Analysis,Phylogenetic Clustering,Phylogenetic Comparative Analysis,Phylogenetic Comparative Methods,Phylogenetic Distance,Phylogenetic Generalized Least Squares,Phylogenetic Groups,Phylogenetic Incongruence,Phylogenetic Inference,Phylogenetic Networks,Phylogenetic Reconstruction,Phylogenetic Relatedness,Phylogenetic Relationships,Phylogenetic Signal,Phylogenetic Structure,Phylogenetic Tree,Phylogenetic Trees,Phylogenomics,Analyse, Phylogenetic,Analysis, Phylogenetic,Analysis, Phylogenetic Comparative,Clustering, Phylogenetic,Community Phylogenetic,Comparative Analysis, Phylogenetic,Comparative Method, Phylogenetic,Distance, Phylogenetic,Group, Phylogenetic,Incongruence, Phylogenetic,Inference, Phylogenetic,Method, Phylogenetic Comparative,Molecular Phylogenetic,Network, Phylogenetic,Phylogenetic Analyse,Phylogenetic Clusterings,Phylogenetic Comparative Analyses,Phylogenetic Comparative Method,Phylogenetic Distances,Phylogenetic Group,Phylogenetic Incongruences,Phylogenetic Inferences,Phylogenetic Network,Phylogenetic Reconstructions,Phylogenetic Relatednesses,Phylogenetic Relationship,Phylogenetic Signals,Phylogenetic Structures,Phylogenetic, Community,Phylogenetic, Molecular,Phylogenies,Phylogenomic,Reconstruction, Phylogenetic,Relatedness, Phylogenetic,Relationship, Phylogenetic,Signal, Phylogenetic,Structure, Phylogenetic,Tree, Phylogenetic
D011418	Prophase	The first phase of cell nucleus division, in which the CHROMOSOMES become visible, the CELL NUCLEUS starts to lose its identity, the SPINDLE APPARATUS appears, and the CENTRIOLES migrate toward opposite poles.	Prophases
D002453	Cell Cycle	The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.	Cell Division Cycle,Cell Cycles,Cell Division Cycles,Cycle, Cell,Cycle, Cell Division,Cycles, Cell,Cycles, Cell Division,Division Cycle, Cell,Division Cycles, Cell
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D016296	Mutagenesis	Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.	Mutageneses
D016547	Kinesins	A family of microtubule-associated mechanical adenosine triphosphatases, that uses the energy of ATP hydrolysis to move organelles along microtubules including mitosis, meiosis, and axonal transport.	Kinesin,Kinesin Heavy-Chain Protein,Kinesin Light-Chain Protein,Kinesin Light-Chain Proteins,Kinesin Superfamily,Heavy-Chain Protein, Kinesin,Light-Chain Protein, Kinesin,Light-Chain Proteins, Kinesin,Protein, Kinesin Heavy-Chain,Protein, Kinesin Light-Chain,Proteins, Kinesin Light-Chain,Superfamily, Kinesin