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Several drugs, containing a halogen atom, F or Br, that are being used in antiviral or anticancer therapy, were studied for their localization in cultured cells by ion microanalysis. The association allows to reduce the exposure time to define the intracellular localization of the studied element. The topography of the cells is given by the image of the polyatomic ion 26CN-. The image of the distribution of 81Br- or 19F-, coded in another color scale, can be superimposed, giving a polychromic image of the cell, thus showing the intracellular localization of the compound. MCF-7 tumor cells were cultured in the presence of pyrimidine derivatives. 5-Bromo-2'-deoxyuridine (BUdR) and 5-trifluorothymidine (F3TdR) were localized in the nucleus, 5-fluoro-2'-deoxyuridine (FUdR) in the nucleus and only in some nucleoli. The method is simple and rapid, as compared with techniques using radiolabeled compounds, or with immunocytochemical techniques. It is possible to observe two different compounds in the same cell. It could be applied to other compounds containing a halogen atom.
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
January 1992,
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
January 1987,
Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
January 1983,
International journal of nuclear medicine and biology,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
November 1985,
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J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
October 1986,
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J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
December 1994,
Journal of microscopy,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
January 1989,
Cell motility and the cytoskeleton,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
January 1980,
Acta haematologica,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
January 1988,
Proteins,
J P Berry, and
G Lespinats, and
F Escaig, and
P Boumati, and
S Tlouzeau, and
J F Cavellier
December 1991,
International journal of experimental pathology,
