Biomaterial Database

Collection of fertilized one-cell mouse eggs for microinjection. 1993

D A Carter

Neuropeptide Laboratory, Institute of Molecular and Cell Biology, National University of Singapore, Republic of Singapore.

Following superovulation and mating, the immature F1 females are killed, and the fertilized F2 eggs are dissected out from the upper part of the oviduct or ampulla. The procedure for egg collection is described in the present chapter. Eggs may be recovered several hours before they are due to be injected. A suitable time for collection would be 11 AM to 12 noon for animals maintained on a light-dark cycle with the mid-dark-phase time at 12 midnight. The procedure is straight-forward, and once the use of the mouth-operated pipet holder is mastered, the vast majority of eggs from 10 mice can be obtained in <1 h.

UI	MeSH Term	Description	Entries
D008845	Microinjections	The injection of very small amounts of fluid, often with the aid of a microscope and microsyringes.	Microinjection
D005260	Female		Females
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D015053	Zygote	The fertilized OVUM resulting from the fusion of a male and a female gamete.	Fertilized Ovum,Ovum, Fertilized,Fertilized Egg,Egg, Fertilized,Eggs, Fertilized,Fertilized Eggs,Zygotes
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D020858	Tissue and Organ Harvesting	The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.	Organ Harvesting,Organ Retrieval,Tissue Harvesting,Organ Harvestings,Organ Retrievals,Retrieval, Organ,Retrievals, Organ