Autologous periodontal ligament cells in the treatment of class II furcation defects: a study in dogs. 2011

Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
Department of Prosthodontics and Periodontics, Division of Periodontics, School of Dentistry at Piracicaba, State University of Campinas, São Paulo, Brazil.

OBJECTIVE The goal of this study was to histologically investigate the use of periodontal ligament cells (PDL cells) in tissue engineering to regenerate class II furcation defects. METHODS PDL cells were obtained from the mandibular tooth extracted from each dog (seven), cultured in vitro and phenotypically characterized with regard to their biological properties. Following, bilateral class II furcation lesions were created at maxillary 3rd premolars and were randomly assigned to the test group [PDL cells+guided tissue regeneration (GTR)] or the control group (GTR). After 3 months, the animals were euthanized to evaluate the histometric parameters. RESULTS In vitro, PDL cells were able to promote mineral nodule formation and to express bone sialoprotein, type I collagen and alkaline phosphatase. Histometrically, data analysis demonstrated that the cell-treated group presented a superior length of new cementum (6.00 ± 1.50 and 8.08 ± 1.08 mm), a greater extension of periodontal regeneration (3.94 ± 1.20 and 7.28 ± 1.00 mm), a lower formation of connective tissue/epithelium (2.15 ± 1.92 and 0.60 ± 0.99 mm), a larger area of new bone (7.01 ± 0.61 and 9.02 ± 2.30 mm(2)) and a smaller area of connective tissue/epithelium (5.90 ± 1.67 and 4.22 ± 0.95 mm(2)), when compared with control group. CONCLUSIONS PDL cells in association with GTR may significantly promote periodontal regeneration in class II furcation defects in dog.

UI MeSH Term Description Entries
D010012 Osteogenesis The process of bone formation. Histogenesis of bone including ossification. Bone Formation,Ossification, Physiologic,Endochondral Ossification,Ossification,Ossification, Physiological,Osteoclastogenesis,Physiologic Ossification,Endochondral Ossifications,Ossification, Endochondral,Ossifications,Ossifications, Endochondral,Osteoclastogeneses,Physiological Ossification
D010513 Periodontal Ligament The fibrous CONNECTIVE TISSUE surrounding the TOOTH ROOT, separating it from and attaching it to the alveolar bone (ALVEOLAR PROCESS). Alveolodental Ligament,Alveolodental Membrane,Gomphosis,Alveolodental Ligaments,Alveolodental Membranes,Gomphoses,Ligament, Alveolodental,Ligament, Periodontal,Membrane, Alveolodental,Periodontal Ligaments
D011897 Random Allocation A process involving chance used in therapeutic trials or other research endeavor for allocating experimental subjects, human or animal, between treatment and control groups, or among treatment groups. It may also apply to experiments on inanimate objects. Randomization,Allocation, Random
D004195 Disease Models, Animal Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases. Animal Disease Model,Animal Disease Models,Disease Model, Animal
D004285 Dogs The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065) Canis familiaris,Dog
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016037 Single-Blind Method A method in which either the observer(s) or the subject(s) is kept ignorant of the group to which the subjects are assigned. Single-Masked Study,Single-Blind Study,Single-Masked Method,Method, Single-Blind,Method, Single-Masked,Methods, Single-Blind,Methods, Single-Masked,Single Blind Method,Single Blind Study,Single Masked Method,Single Masked Study,Single-Blind Methods,Single-Blind Studies,Single-Masked Methods,Single-Masked Studies,Studies, Single-Blind,Studies, Single-Masked,Study, Single-Blind,Study, Single-Masked
D016557 Guided Tissue Regeneration, Periodontal Techniques for enhancing and directing cell growth to repopulate specific parts of the PERIODONTIUM that have been damaged by PERIODONTAL DISEASES; TOOTH DISEASES; or TRAUMA, or to correct TOOTH ABNORMALITIES. Repopulation and repair is achieved by guiding the progenitor cells to reproduce in the desired location by blocking contact with surrounding tissue by use of membranes composed of synthetic or natural material that may include growth inducing factors as well. Guided Periodontal Tissue Regeneration,Periodontal Guided Tissue Regeneration,Regeneration, Periodontal Guided Tissue
D017690 Cell Transplantation Transference of cells within an individual, between individuals of the same species, or between individuals of different species. Transplantation, Cell
D054457 Tissue Scaffolds Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses. Tissue Scaffolding,Scaffold, Tissue,Scaffolding, Tissue,Scaffoldings, Tissue,Scaffolds, Tissue,Tissue Scaffold,Tissue Scaffoldings

Related Publications

Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
April 2005, Journal of periodontology,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
April 1988, Journal of clinical periodontology,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
June 1991, Journal of periodontology,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
June 2006, Biomedical research (Tokyo, Japan),
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
January 2012, Journal of applied oral science : revista FOB,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
May 2018, Journal of biomedical materials research. Part B, Applied biomaterials,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
July 2010, Cytotechnology,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
March 1990, Journal of clinical periodontology,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
December 2017, Journal of periodontal & implant science,
Fabricia Ferreira Suaid, and Fernanda Vieira Ribeiro, and Thaisângela L Rodrigues, and Karina Gonzales Silvério, and Marcelo Diniz Carvalho, and Francisco Humberto Nociti, and Marcio Zaffalon Casati, and Enilson Antônio Sallum
May 1997, Journal of periodontology,
Copied contents to your clipboard!