A survey of parasites of freshwater fishes in Harike and Ropar Wetlands of Punjab (India) revealed the presence of two new myxosporean species belonging to the genus Myxobolus Butschli 1882. Spores of the first species, M. duodenalis sp. nov. parasitize the inner wall of the duodenum of Wallago attu (Bloch and Schneider) (Cypriniformis: Cyprinidae) vern. mulli are characterized by a broad, pyriform shape with a blunt anterior end and measures 9.0 × 3.20 μm. Spore valves are thin, smooth, and symmetrical with no parietal folds. There are two polar capsules, prominently unequal and pyriform in shape, with a narrow anterior end and a rounded posterior end. The larger polar capsule measures 4.70 × 1.76 μm and the smaller 2.76 × 1.06 μm. The larger polar capsule occupies more than half, while the smaller one occupies one third of the spore body cavity. An intercapsular process is absent. Spores of the second species, Myxobolus patialensis sp. nov. parasitize the caudal fin of Labeo rohita (Ham. 1822) (Cypriniformis: Cyprinidae) vern. rohu are pyriform in valvular view, measuring 11.28 × 6.67 μm. The two shell valves are asymmetrical and contain five parietal folds along the posterior end of the spore. Two anteriorly situated polar capsules, elongated and oval in shape are prominently unequal in size. The larger polar capsule measures 4.8 × 3.1 μm and smaller one 1.70 × 1.51 μm. The larger polar capsule lies obliquely to the spore axis and the smaller one is at the same level but pointing outward anteriolaterally. An intercapsular process is absent. This species is characterized by having a prominent ridge on the shell surface anteriolaterally on the side of the smaller polar capsule.