-transparent.png){kind=logo}
We investigated the occurrence and subcellular localization of chromogranins A and B in atrial myoendocrine cells of rat heart, using immunological methods. Immunoblotting revealed the presence of both chromogranin A and B in an extract from large granules isolated from this tissue by subcellular fractionation. Immunohistochemistry at the ultrastructural level demonstrated the presence of chromogranin A and B in secretory granules. These organelles also immunostained for atrial natriuretic peptides (ANP). Within a given section, all granules were labeled with immunogold for these three antigens. This apparent co-localization of the three antigens was confirmed by double immunostaining with immunogold particles of different sizes. We conclude that, in agreement with their endocrine nature, the secretory organelles of rat atria contain both chromogranins A and B. Apparently these acidic peptides, which have a widespread distribution in the endocrine system, are co-stored and therefore also co-secreted with ANP.
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
March 1989,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
June 1985,
Journal of neurochemistry,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
January 2018,
Heart failure clinics,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
December 1990,
Endocrinology,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
January 2000,
Advances in experimental medicine and biology,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
December 2009,
Medical molecular morphology,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
April 2000,
The Journal of biological chemistry,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
January 2005,
FEBS letters,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
June 2007,
Neuroscience letters,
H J Steiner, and
R Weiler, and
C Ludescher, and
K W Schmid, and
H Winkler
January 2000,
Advances in experimental medicine and biology,
