Direct regulation of Escherichia coli ribosomal protein promoters by the transcription factors ppGpp and DksA. 2011

Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
Department of Bacteriology, University of Wisconsin, Madison, WI 53719, USA.

We show here that the promoters for many of the Escherichia coli ribosomal protein operons are regulated directly by two transcription factors, the small RNA polymerase-binding protein DksA and the nutritional stress-induced nucleotide ppGpp. ppGpp and DksA work together to inhibit transcription initiation from ribosomal protein promoters in vitro and in vivo. The degree of promoter regulation by ppGpp/DksA varies among the r-protein promoters, but some are inhibited almost as much as rRNA promoters. Thus, many r-protein operons are regulated at the level of transcription in addition to their control by the classic translational feedback systems discovered ~30 y ago. We conclude that direct control of r-protein promoters and rRNA promoters by the same signal, ppGpp/DksA, makes a major contribution to the balanced and coordinated synthesis rates of all of the ribosomal components.

UI MeSH Term Description Entries
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011401 Promoter Regions, Genetic DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes. rRNA Promoter,Early Promoters, Genetic,Late Promoters, Genetic,Middle Promoters, Genetic,Promoter Regions,Promoter, Genetic,Promotor Regions,Promotor, Genetic,Pseudopromoter, Genetic,Early Promoter, Genetic,Genetic Late Promoter,Genetic Middle Promoters,Genetic Promoter,Genetic Promoter Region,Genetic Promoter Regions,Genetic Promoters,Genetic Promotor,Genetic Promotors,Genetic Pseudopromoter,Genetic Pseudopromoters,Late Promoter, Genetic,Middle Promoter, Genetic,Promoter Region,Promoter Region, Genetic,Promoter, Genetic Early,Promoter, rRNA,Promoters, Genetic,Promoters, Genetic Middle,Promoters, rRNA,Promotor Region,Promotors, Genetic,Pseudopromoters, Genetic,Region, Genetic Promoter,Region, Promoter,Region, Promotor,Regions, Genetic Promoter,Regions, Promoter,Regions, Promotor,rRNA Promoters
D011755 Pyrophosphatases A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-. Pyrophosphatase
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D001616 beta-Galactosidase A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1. Lactases,Dairyaid,Lactaid,Lactogest,Lactrase,beta-D-Galactosidase,beta-Galactosidase A1,beta-Galactosidase A2,beta-Galactosidase A3,beta-Galactosidases,lac Z Protein,Protein, lac Z,beta D Galactosidase,beta Galactosidase,beta Galactosidase A1,beta Galactosidase A2,beta Galactosidase A3,beta Galactosidases
D012269 Ribosomal Proteins Proteins found in ribosomes. They are believed to have a catalytic function in reconstituting biologically active ribosomal subunits. Proteins, Ribosomal,Ribosomal Protein,Protein, Ribosomal
D014157 Transcription Factors Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. Transcription Factor,Factor, Transcription,Factors, Transcription
D015964 Gene Expression Regulation, Bacterial Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria. Bacterial Gene Expression Regulation,Regulation of Gene Expression, Bacterial,Regulation, Gene Expression, Bacterial
D029968 Escherichia coli Proteins Proteins obtained from ESCHERICHIA COLI. E coli Proteins

Related Publications

Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
May 2005, Proceedings of the National Academy of Sciences of the United States of America,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
December 2009, Molecular microbiology,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
October 2010, Molecular genetics and genomics : MGG,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
October 2011, Microbiology (Reading, England),
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
May 2009, Journal of bacteriology,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
January 1992, Journal of molecular biology,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
August 2004, Cell,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
June 2006, The Journal of biological chemistry,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
July 2007, Journal of bacteriology,
Justin J Lemke, and Patricia Sanchez-Vazquez, and Hector L Burgos, and Gina Hedberg, and Wilma Ross, and Richard L Gourse
January 2009, The Journal of biological chemistry,
Copied contents to your clipboard!