Molecular cloning, expression, and immunolocalization of the NAD(+)-dependent glycerol 3-phosphate dehydrogenase (GPD) from Clonorchis sinensis. 2011

Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, People's Republic of China.

Glycerol 3-phosphate dehydrogenase (GPD) plays an important role in the energy metabolism and nutrition metabolism. In order to know about the biological functions of GPD of Clonorchis sinensis (C. sinensis), we identified a complete gene coding GPD from C. sinensis metacercaria cDNA library. This novel cDNA sequence contains 1,056 bp with a putative open reading frame of 351 amino acids and shares 74% identity with GPD from Schistosoma mansoni. Recombinant CsGPD was expressed and purified from Escherichia coli BL21 (DE3). Western blot analysis displayed that recombinant CsGPD can be recognized by anti-CsGPD serum and C. sinensis-infected serum. RT-PCR and immunolocalization analysis confirmed that GPD expressed both at the stage of adult worm and metacercaria of C. sinensis and immunolocated at the tegument of adult worm, tegument and tegumentary cells of metacercaria. Our current study has paved the way for the further researches about the biological functions involved in the growth of C. sinensis.

UI MeSH Term Description Entries
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D003004 Clonorchis sinensis A species of trematode flukes of the family Opisthorchidae. Many authorities consider this genus belonging to Opisthorchis. It is common in China and other Asiatic countries. Snails and fish are the intermediate hosts. Opisthorchis sinensis,Clonorchis sinenses,Opisthorchis sinenses,sinenses, Opisthorchis,sinensis, Clonorchis
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000909 Antibodies, Helminth Immunoglobulins produced in a response to HELMINTH ANTIGENS. Helminth Antibodies
D015151 Immunoblotting Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies. Dot Immunoblotting,Electroimmunoblotting,Immunoelectroblotting,Reverse Immunoblotting,Immunoblotting, Dot,Immunoblotting, Reverse,Dot Immunoblottings,Electroimmunoblottings,Immunoblottings,Immunoblottings, Dot,Immunoblottings, Reverse,Immunoelectroblottings,Reverse Immunoblottings
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D016366 Open Reading Frames A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR). ORFs,Protein Coding Region,Small Open Reading Frame,Small Open Reading Frames,sORF,Unassigned Reading Frame,Unassigned Reading Frames,Unidentified Reading Frame,Coding Region, Protein,Frame, Unidentified Reading,ORF,Open Reading Frame,Protein Coding Regions,Reading Frame, Open,Reading Frame, Unassigned,Reading Frame, Unidentified,Region, Protein Coding,Unidentified Reading Frames

Related Publications

Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
January 2004, Yeast (Chichester, England),
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
May 1995, Biochimica et biophysica acta,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
January 1996, Molecular and biochemical parasitology,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
August 2008, FEMS yeast research,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
September 2012, Parasitology research,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
August 2003, Parasitology research,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
April 2005, Experimental parasitology,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
January 2013, PloS one,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
April 2008, Parasitology research,
Yongxiu Fan, and Xiaoyun Wang, and Chuanhuan Deng, and Yan Huang, and Lexun Wang, and Wenjun Chen, and Chi Liang, and Xuerong Li, and Zhongdao Wu, and Xinbing Yu
August 2011, Parasitology research,
Copied contents to your clipboard!