Pinocembrin attenuates blood-brain barrier injury induced by global cerebral ischemia-reperfusion in rats. 2011

Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, P.R. China.

Blood-brain barrier (BBB) disruption is a major consequence of cerebral ischemia/reperfusion. Several studies have reported the neuroprotection of pinocembrin on cerebral ischemia in vivo and in vitro, but the effects of pinocembrin on BBB and its underlying mechanisms are not clear. In this study, we investigated the effects of pinocembrin on BBB functions in the global cerebral ischemia/reperfusion (GCI/R) model in rats. Neurological scores and brain edema were evaluated. BBB permeability was assessed by detecting the concentrations of Evan's blue (EB) and fluorescein sodium (NaF) in brain tissue. The pathological changes of BBB ultrastructure were observed by transmission electron microscopy. Cerebral blood flow (CBF) was measured by laser Doppler flowmetry. The effects of pinocembrin on primary cultured rat cerebral microvascular endothelial cells (RCMECs) against oxygen-glucose deprivation/reoxygenation (OGD/R) were also investigated. The results showed pinocembrin decreased neurological score and lessened brain edema induced by GCI/R. Pinocembrin also reduced the concentrations of EB and NaF in brain tissue of the GCI/R rats. And pinocembrin alleviated the ultrastructural changes of cerebral microvessels, astrocyte end-feet and neurons, and improved CBF in the GCI/R rats. In addition, pinocembrin increased the viability and mitochondrial membrane potential of cultured RCMECs induced by OGD/R. In conclusion, these data demonstrate that pinocembrin alleviates blood-brain barrier injury induced by GCI/R in rats.

UI MeSH Term Description Entries
D008297 Male Males
D009460 Neurologic Examination Assessment of sensory and motor responses and reflexes that is used to determine impairment of the nervous system. Examination, Neurologic,Neurological Examination,Examination, Neurological,Examinations, Neurologic,Examinations, Neurological,Neurologic Examinations,Neurological Examinations
D010100 Oxygen An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration. Dioxygen,Oxygen-16,Oxygen 16
D010539 Permeability Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions. Permeabilities
D012039 Regional Blood Flow The flow of BLOOD through or around an organ or region of the body. Blood Flow, Regional,Blood Flows, Regional,Flow, Regional Blood,Flows, Regional Blood,Regional Blood Flows
D001794 Blood Pressure PRESSURE of the BLOOD on the ARTERIES and other BLOOD VESSELS. Systolic Pressure,Diastolic Pressure,Pulse Pressure,Pressure, Blood,Pressure, Diastolic,Pressure, Pulse,Pressure, Systolic,Pressures, Systolic
D001812 Blood-Brain Barrier Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue. Brain-Blood Barrier,Hemato-Encephalic Barrier,Barrier, Blood-Brain,Barrier, Brain-Blood,Barrier, Hemato-Encephalic,Barriers, Blood-Brain,Barriers, Brain-Blood,Barriers, Hemato-Encephalic,Blood Brain Barrier,Blood-Brain Barriers,Brain Blood Barrier,Brain-Blood Barriers,Hemato Encephalic Barrier,Hemato-Encephalic Barriers
D001929 Brain Edema Increased intracellular or extracellular fluid in brain tissue. Cytotoxic brain edema (swelling due to increased intracellular fluid) is indicative of a disturbance in cell metabolism, and is commonly associated with hypoxic or ischemic injuries (see HYPOXIA, BRAIN). An increase in extracellular fluid may be caused by increased brain capillary permeability (vasogenic edema), an osmotic gradient, local blockages in interstitial fluid pathways, or by obstruction of CSF flow (e.g., obstructive HYDROCEPHALUS). (From Childs Nerv Syst 1992 Sep; 8(6):301-6) Brain Swelling,Cerebral Edema,Cytotoxic Brain Edema,Intracranial Edema,Vasogenic Cerebral Edema,Cerebral Edema, Cytotoxic,Cerebral Edema, Vasogenic,Cytotoxic Cerebral Edema,Vasogenic Brain Edema,Brain Edema, Cytotoxic,Brain Edema, Vasogenic,Brain Swellings,Cerebral Edemas, Vasogenic,Edema, Brain,Edema, Cerebral,Edema, Cytotoxic Brain,Edema, Cytotoxic Cerebral,Edema, Intracranial,Edema, Vasogenic Brain,Edema, Vasogenic Cerebral,Swelling, Brain
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002545 Brain Ischemia Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION. Cerebral Ischemia,Ischemic Encephalopathy,Encephalopathy, Ischemic,Ischemia, Cerebral,Brain Ischemias,Cerebral Ischemias,Ischemia, Brain,Ischemias, Cerebral,Ischemic Encephalopathies

Related Publications

Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
October 2005, Brain research,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
July 2005, Brain research,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
September 2016, Steroids,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
January 2015, Experimental & translational stroke medicine,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
April 2021, Journal of cardiovascular translational research,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
March 2009, European journal of pharmacology,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
August 1999, Zhongguo yao li xue bao = Acta pharmacologica Sinica,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
July 2022, Annals of translational medicine,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
March 2022, Journal of Asian natural products research,
Fanrui Meng, and Rui Liu, and Mei Gao, and Yuehua Wang, and Xiaoyan Yu, and Zhaohong Xuan, and Jialin Sun, and Fan Yang, and Chunfu Wu, and Guanhua Du
June 2015, Scientific reports,
Copied contents to your clipboard!