Biomaterial Database

Potency of detergents in enhancing Schistosoma mansoni tegumental antigens. 2011

Manall Abdel Aziz Hamed

Therapeutic Chemistry Department, National Research Center, Cairo, Egypt. manal_hamed@yahoo.com

BACKGROUND Vaccine strategies represent an essential component for the future control of schistosomiasis. This work aimed to evaluate the role of detergents for potentiating the effect of Schistosoma mansoni tegumental antigens (TA) against challenge infection. METHODS Two detergents; Triton X-100 (TX-100) and sodium dodecyl sulfate (SDS), were selected for extraction of tegumental proteins. Mice were vaccinated with two doses of each preparation (100 µg protein) at time intervals 15 days before infection. Evaluation was performed by estimating particular metabolic pathways, including the Krebs cycle (via succinate dehydrogenase); glycolysis (lactate dehydrogenase); gluconeogenesis (glucose-6-phosphatase); hydrolytic enzymes (acid phosphatase); and nucleic acid catabolic enzymes (5'-nucleotidase). Serum protein profiles, worm burden, ova counts, spleen and relative liver weights were also investigated. The work was extended to study the histopathological picture of the liver including granuloma count, and total infection area. Vaccinated post-challenged mice showed amelioration of the selected biochemical parameters and reduction in worm and ova count (P ≤ 0.0001) as well as improvement in the histological features of the liver. RESULTS Triton X-100 potentiated the protective effect of S. mansoni tegumental antigen and extracted effective proteins better than SDS, while TA alone recorded the lowest level of protection. CONCLUSIONS TX-100 will be more efficient for future studies in molecular identification of novel candidate tegumental proteins.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D008853	Microscopy	The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.	Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D010270	Parasite Egg Count	Determination of parasite eggs in feces.	Count, Parasite Egg,Counts, Parasite Egg,Egg Count, Parasite,Egg Counts, Parasite,Parasite Egg Counts
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D003902	Detergents	Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.	Cleansing Agents,Detergent Pods,Laundry Detergent Pods,Laundry Pods,Syndet,Synthetic Detergent,Agent, Cleansing,Agents, Cleansing,Cleansing Agent,Detergent,Detergent Pod,Detergent Pod, Laundry,Detergent Pods, Laundry,Detergent, Synthetic,Detergents, Synthetic,Laundry Detergent Pod,Laundry Pod,Pod, Detergent,Pod, Laundry,Pod, Laundry Detergent,Pods, Detergent,Pods, Laundry,Pods, Laundry Detergent,Synthetic Detergents
D004195	Disease Models, Animal	Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases.	Animal Disease Model,Animal Disease Models,Disease Model, Animal
D006099	Granuloma	A relatively small nodular inflammatory lesion containing grouped mononuclear phagocytes, caused by infectious and noninfectious agents.	Granulomas
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia