OBJECTIVE To evaluate the clinical effect of root canal filling with warm vertical condensation with or without dental operating microscope. METHODS Fifty maxillary anterior teeth with single, straight canals were divided into two groups. Each tooth was instrumented by X-Smart rotary nickel-titanium files to a master file 0.06 taper F3, root canal in the group 1 was obturated with warm vertical condensation using system B for downpack and Obtura II for backfilling without dental operating microscope; root canal in the group 2 was obturated using the same methods under the dental operating microscope. The effect of quality of root canal filling was evaluated by X-ray radiograph post-operatively and after two years of treatment. SPSS10.0 software package was used for Chi-square test. RESULTS Significant difference was found between the two groups on the quality of root canal filling post-operatively (P < 0.05),with better quality of root canal filling in the group using dental operating microscope, no significant difference was found between the two groups after two years (P > 0.05). CONCLUSIONS Warm vertical condensation under the dental operating microscope is a good method for root canal filling. It may improve the quality of root canal filling effectively, and therefore, worthy of clinical application.