Steady state migratory RelB+ langerin+ dermal dendritic cells mediate peripheral induction of antigen-specific CD4+ CD25+ Foxp3+ regulatory T cells. 2011

Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.

Tolerance to self-antigens expressed in peripheral organs is maintained by CD4(+) CD25(+) Foxp3(+) Treg cells, which are generated as a result of thymic selection or peripheral induction. Here, we demonstrate that steady-state migratory DCs from the skin mediated Treg conversion in draining lymph nodes of mice. These DCs displayed a partially mature MHC II(int) CD86(int) CD40(hi) CCR7(+) phenotype, used endogenous TGF-β for conversion and showed nuclear RelB translocation. Deficiency of the alternative NF-κB signaling pathway (RelB/p52) reduced steady-state migration of DCs. These DCs transported and directly presented soluble OVA provided by s.c. implanted osmotic minipumps, as well as cell-associated epidermal OVA in transgenic K5-mOVA mice to CD4(+) OVA-specific TCR-transgenic OT-II T cells. The langerin(+) dermal DC subset, but not epidermal Langerhans cells, mediated conversion of naive OT-II×RAG-1(-/-) T cells into proliferating CD4(+) CD25(+) Foxp3(+) Tregs. Thus, our data suggest that steady-state migratory RelB(+) TGF-β(+) langerin(+) dermal DCs mediate peripheral Treg conversion in response to epidermal antigen in skin-draining lymph nodes.

UI MeSH Term Description Entries
D007801 Langerhans Cells Recirculating, dendritic, antigen-presenting cells containing characteristic racket-shaped granules (Birbeck granules). They are found principally in the stratum spinosum of the EPIDERMIS and are rich in Class II MAJOR HISTOCOMPATIBILITY COMPLEX molecules. Langerhans cells were the first dendritic cell to be described and have been a model of study for other dendritic cells (DCs), especially other migrating DCs such as dermal DCs and INTERSTITIAL DENDRITIC CELLS. Langerhans Cell,Dendritic Cells, Dermal,Dendritic Cells, Epidermal,Dendritic Cells, Skin,Dermal Dendritic Cells,Epidermal Dendritic Cells,Skin Dendritic Cells,Cell, Dermal Dendritic,Cell, Epidermal Dendritic,Cell, Langerhans,Cell, Skin Dendritic,Cells, Dermal Dendritic,Cells, Epidermal Dendritic,Cells, Langerhans,Cells, Skin Dendritic,Dendritic Cell, Dermal,Dendritic Cell, Epidermal,Dendritic Cell, Skin,Dermal Dendritic Cell,Epidermal Dendritic Cell,Skin Dendritic Cell
D008198 Lymph Nodes They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system. Lymph Node,Node, Lymph,Nodes, Lymph
D008285 Major Histocompatibility Complex The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement. Histocompatibility Complex,Complex, Histocompatibility,Complex, Major Histocompatibility,Complices, Histocompatibility,Complices, Major Histocompatibility,Histocompatibility Complex, Major,Histocompatibility Complices,Histocompatibility Complices, Major,Major Histocompatibility Complices
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008822 Mice, Transgenic Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN. Transgenic Mice,Founder Mice, Transgenic,Mouse, Founder, Transgenic,Mouse, Transgenic,Mice, Transgenic Founder,Transgenic Founder Mice,Transgenic Mouse
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005455 Fluorescent Antibody Technique Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy. Antinuclear Antibody Test, Fluorescent,Coon's Technique,Fluorescent Antinuclear Antibody Test,Fluorescent Protein Tracing,Immunofluorescence Technique,Coon's Technic,Fluorescent Antibody Technic,Immunofluorescence,Immunofluorescence Technic,Antibody Technic, Fluorescent,Antibody Technics, Fluorescent,Antibody Technique, Fluorescent,Antibody Techniques, Fluorescent,Coon Technic,Coon Technique,Coons Technic,Coons Technique,Fluorescent Antibody Technics,Fluorescent Antibody Techniques,Fluorescent Protein Tracings,Immunofluorescence Technics,Immunofluorescence Techniques,Protein Tracing, Fluorescent,Protein Tracings, Fluorescent,Technic, Coon's,Technic, Fluorescent Antibody,Technic, Immunofluorescence,Technics, Fluorescent Antibody,Technics, Immunofluorescence,Technique, Coon's,Technique, Fluorescent Antibody,Technique, Immunofluorescence,Techniques, Fluorescent Antibody,Techniques, Immunofluorescence,Tracing, Fluorescent Protein,Tracings, Fluorescent Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000954 Antigens, Surface Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated. Cell Surface Antigens,Surface Antigens,Surface Markers, Immunological,Cell Surface Antigen,Immunologic Surface Markers,Markers, Immunological Surface,Surface Antigen,Surface Markers, Immunologic,Antigen, Cell Surface,Antigen, Surface,Antigens, Cell Surface,Immunological Surface Markers,Markers, Immunologic Surface,Surface Antigen, Cell,Surface Antigens, Cell

Related Publications

Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
January 2017, Frontiers in immunology,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
August 2006, Immunological reviews,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
October 2015, International immunopharmacology,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
December 2003, The Journal of experimental medicine,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
February 2012, Immunology and cell biology,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
May 2008, Journal of leukocyte biology,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
December 2006, Journal of immunology (Baltimore, Md. : 1950),
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
October 2007, Journal of immunology (Baltimore, Md. : 1950),
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
January 2012, Transplant immunology,
Hiroaki Azukizawa, and Anja Döhler, and Nobuo Kanazawa, and Arnab Nayak, and Martin Lipp, and Bernard Malissen, and Ingo Autenrieth, and Ichiro Katayama, and Marc Riemann, and Falk Weih, and Friederike Berberich-Siebelt, and Manfred B Lutz
December 2004, Journal of immunology (Baltimore, Md. : 1950),
Copied contents to your clipboard!