Serum calcium-decreasing factor, caldecrin, ameliorates muscular dystrophy in dy/dy mice. 2011

Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
Division of Biochemistry, Department of Oral Biology and Tissue Engineering, Meikai University School of Dentistry, 1-1 Keyakidai, Sakado, Saitama 350-0283 Japan. atomomu@dent.meikai.ac.jp

BACKGROUND Calcium signaling is important in muscular cells and abnormal Ca(2+) handling results in muscle damage. Caldecrin is a serum calcium-decreasing factor purified from pancreas. It is a chymotrypsin-type secretory protease, whereas the serum calcium-decreasing activity does not depend on its protease activity. Here, we evaluated the effect of caldecrin on dystrophia muscularis (dy/dy) mice. METHODS Caldecrin gene in hemagglutinating virus of Japan envelop vector (10 μg/head) was single injected in the femoral muscle or daily intraperitoneal administration of caldecrin (100 μg/kg body weight) was given for 4 days in dy/dy mice. Progression of muscular dystrophy was assessed by blood urea nitrogen and Evans blue dye penetration assay. RESULTS Ectopic expression of the caldecrin gene in the muscle of dy/dy mice reduced the level of blood urea nitrogen and improved the dystrophic progression similarly to that of caldecrin-injected mice. CONCLUSIONS Caldecrin treatment is effective for preventing muscular dystrophy.

UI MeSH Term Description Entries
D007273 Injections, Intramuscular Forceful administration into a muscle of liquid medication, nutrient, or other fluid through a hollow needle piercing the muscle and any tissue covering it. Intramuscular Injections,Injection, Intramuscular,Intramuscular Injection
D007274 Injections, Intraperitoneal Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall. Intraperitoneal Injections,Injection, Intraperitoneal,Intraperitoneal Injection
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008813 Mice, Inbred ICR An inbred strain of mouse that is used as a general purpose research strain, for therapeutic drug testing, and for the genetic analysis of CARCINOGEN-induced COLON CANCER. Mice, Inbred ICRC,Mice, ICR,Mouse, ICR,Mouse, Inbred ICR,Mouse, Inbred ICRC,ICR Mice,ICR Mice, Inbred,ICR Mouse,ICR Mouse, Inbred,ICRC Mice, Inbred,ICRC Mouse, Inbred,Inbred ICR Mice,Inbred ICR Mouse,Inbred ICRC Mice,Inbred ICRC Mouse
D009137 Muscular Dystrophy, Animal MUSCULAR DYSTROPHY that occurs in VERTEBRATE animals. Animal Muscular Dystrophies,Animal Muscular Dystrophy,Dystrophies, Animal Muscular,Dystrophy, Animal Muscular,Muscular Dystrophies, Animal
D001806 Blood Urea Nitrogen The urea concentration of the blood stated in terms of nitrogen content. Serum (plasma) urea nitrogen is approximately 12% higher than blood urea nitrogen concentration because of the greater protein content of red blood cells. Increases in blood or serum urea nitrogen are referred to as azotemia and may have prerenal, renal, or postrenal causes. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984) BUN,Nitrogen, Blood Urea,Urea Nitrogen, Blood
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D005260 Female Females
D005822 Genetic Vectors DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition. Cloning Vectors,Shuttle Vectors,Vectors, Genetic,Cloning Vector,Genetic Vector,Shuttle Vector,Vector, Cloning,Vector, Genetic,Vector, Shuttle,Vectors, Cloning,Vectors, Shuttle

Related Publications

Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
January 2002, Neuroscience letters,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
December 1995, The Journal of biological chemistry,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
August 2010, The Journal of biological chemistry,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
November 2001, FEBS letters,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
December 1993, FEBS letters,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
April 1992, FEBS letters,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
January 2016, The Journal of biological chemistry,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
January 1964, Enzymologia biologica et clinica,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
October 2003, Pediatric research,
Mineko Tomomura, and Takeru Fujii, and Hiroshi Sakagami, and Akito Tomomura
December 2012, Proceedings of the National Academy of Sciences of the United States of America,
Copied contents to your clipboard!