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Spin labeled poly rA (sl-poly rA) was encapsulated by the coat proteins of two plant viruses having different morphologies: TMV, a rigid rod and CCMV, an icosahedral sphere. Electron microscopy showed that the resultant particles were morphologically similar to the parent virus from which the coat protein was obtained. Encapsulation produced progressive immobilization of the spin label. The motion of the spin label attached to TMV-sl-poly rA appears anisotropic with a correlation time about the long axis of approximately 5 x 10(-6) sec. Exogenous nuclease had no effect on the epr spectrum of this nucleo-protein complex. The epr spectrum of CCMV-sl-poly rA was isotropic with a correlation time less than 5 x 10(-7). CCMV-sl-poly rA was partially degraded by T2 ribonuclease. Theoretical calculations of correlation times for the motion of the nucleo-protein particles were similar to the experimentally derived values suggesting that the nucleo-protein particles are tightly packed with little potential for internal motion.
A T Powell, and
M P Gordon, and
W J Caspary, and
J J Greene, and
P O Ts'o
April 1976,
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A T Powell, and
M P Gordon, and
W J Caspary, and
J J Greene, and
P O Ts'o
September 1967,
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A T Powell, and
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September 1970,
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A T Powell, and
M P Gordon, and
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P O Ts'o
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A T Powell, and
M P Gordon, and
W J Caspary, and
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P O Ts'o
January 1972,
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A T Powell, and
M P Gordon, and
W J Caspary, and
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P O Ts'o
February 1973,
Biochimica et biophysica acta,
A T Powell, and
M P Gordon, and
W J Caspary, and
J J Greene, and
P O Ts'o
July 1964,
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A T Powell, and
M P Gordon, and
W J Caspary, and
J J Greene, and
P O Ts'o
September 1966,
Biochimica et biophysica acta,
A T Powell, and
M P Gordon, and
W J Caspary, and
J J Greene, and
P O Ts'o
May 1959,
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A T Powell, and
M P Gordon, and
W J Caspary, and
J J Greene, and
P O Ts'o
September 1951,
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