New markers for minimal residual disease detection in acute lymphoblastic leukemia. 2011

Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
Department of Oncology, St. Jude Children’s Research Hospital, Memphis, TN, USA.

To identify new markers for minimal residual disease (MRD) detection in acute lymphoblastic leukemia (ALL), we compared genome-wide gene expression of lymphoblasts from 270 patients with newly diagnosed childhood ALL to that of normal CD19⁺CD10⁺ B-cell progenitors (n = 4). Expression of 30 genes differentially expressed by ≥ 3-fold in at least 25% of cases of ALL (or 40% of ALL subtypes) was tested by flow cytometry in 200 B-lineage ALL and 61 nonleukemic BM samples, including samples containing hematogones. Of the 30 markers, 22 (CD44, BCL2, HSPB1, CD73, CD24, CD123, CD72, CD86, CD200, CD79b, CD164, CD304, CD97, CD102, CD99, CD300a, CD130, PBX1, CTNNA1, ITGB7, CD69, CD49f) were differentially expressed in up to 81.4% of ALL cases; expression of some markers was associated with the presence of genetic abnormalities. Results of MRD detection by flow cytometry with these markers correlated well with those of molecular testing (52 follow-up samples from 18 patients); sequential studies during treatment and diagnosis-relapse comparisons documented their stability. When incorporated in 6-marker combinations, the new markers afforded the detection of 1 leukemic cell among 10(5) BM cells. These new markers should allow MRD studies in all B-lineage ALL patients, and substantially improve their sensitivity.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D008297 Male Males
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D005260 Female Females
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D014408 Biomarkers, Tumor Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or BODY FLUIDS. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including HORMONES; ANTIGENS; amino and NUCLEIC ACIDS; ENZYMES; POLYAMINES; and specific CELL MEMBRANE PROTEINS and LIPIDS. Biochemical Tumor Marker,Cancer Biomarker,Carcinogen Markers,Markers, Tumor,Metabolite Markers, Neoplasm,Tumor Biomarker,Tumor Marker,Tumor Markers, Biochemical,Tumor Markers, Biological,Biochemical Tumor Markers,Biological Tumor Marker,Biological Tumor Markers,Biomarkers, Cancer,Marker, Biochemical Tumor,Marker, Biologic Tumor,Marker, Biological Tumor,Marker, Neoplasm Metabolite,Marker, Tumor Metabolite,Markers, Biochemical Tumor,Markers, Biological Tumor,Markers, Neoplasm Metabolite,Markers, Tumor Metabolite,Metabolite Markers, Tumor,Neoplasm Metabolite Markers,Tumor Markers, Biologic,Tumor Metabolite Marker,Biologic Tumor Marker,Biologic Tumor Markers,Biomarker, Cancer,Biomarker, Tumor,Cancer Biomarkers,Marker, Tumor,Markers, Biologic Tumor,Markers, Carcinogen,Metabolite Marker, Neoplasm,Metabolite Marker, Tumor,Neoplasm Metabolite Marker,Tumor Biomarkers,Tumor Marker, Biochemical,Tumor Marker, Biologic,Tumor Marker, Biological,Tumor Markers,Tumor Metabolite Markers

Related Publications

Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
February 2020, International journal of molecular sciences,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
August 1995, Blood,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
October 2001, Onkologie,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
January 2013, Cytometry. Part B, Clinical cytometry,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
August 2016, Journal of pediatric hematology/oncology,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
October 1990, Hematology/oncology clinics of North America,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
January 2009, Seminars in hematology,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
January 2010, Hematology. American Society of Hematology. Education Program,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
April 2001, Blood,
Elaine Coustan-Smith, and Guangchun Song, and Christopher Clark, and Laura Key, and Peixin Liu, and Mohammad Mehrpooya, and Patricia Stow, and Xiaoping Su, and Sheila Shurtleff, and Ching-Hon Pui, and James R Downing, and Dario Campana
December 2012, Blood,
Copied contents to your clipboard!