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A method using the principle of affinity elution chromatography is described for the assay of adenylate cyclase in intact human platelets. By incubating platelet-rich plasma in the presence of radioactively labelled adenine, the ATP pool of the cells was prelabelled. Formation of labelled cyclic AMP from ATP was determined by extracting the platelets with HC1O4. After removal of the latter as KC1O4, the extract containing cyclic AMP and other adenine nucleotides was adsorbed in a NN-diethyl-N-2-hydroxypropylamino (QAE)-cellulose column. The column was washed, and subsequently cyclic AMP was specifically eluted with a cyclic AMP-dependent protein kinase and the radioactivity of the eluate was determined.
A K Sinha, and
R W Colman
October 1974,
Analytical biochemistry,
A K Sinha, and
R W Colman
May 1993,
Analytical biochemistry,
A K Sinha, and
R W Colman
January 1987,
The American journal of physiology,
A K Sinha, and
R W Colman
January 1979,
Advances in cyclic nucleotide research,
A K Sinha, and
R W Colman
November 1969,
Analytical biochemistry,
A K Sinha, and
R W Colman
August 1980,
Analytical biochemistry,
A K Sinha, and
R W Colman
April 1974,
Analytical biochemistry,
A K Sinha, and
R W Colman
March 1979,
FEBS letters,
A K Sinha, and
R W Colman
October 1987,
Analytical biochemistry,
A K Sinha, and
R W Colman
April 1980,
The Biochemical journal,
